Ethanolic Extract of Mangifera indica Protects against CCl4-Induced Hepatotoxicity via Antioxidant Capabilities in Albino Rats.

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2024-08-27 eCollection Date: 2024-01-01 DOI:10.1155/2024/5539386
Ahmed Saeed Kabbashi, Salwa Abdulla Eltawaty, Amar Mohamed Ismail, Ahmed Abdelhhafiz Elshikh, Ayat Ahmed Alrasheid, Rawan Ahmed Elmahi, Waleed S Koko, Elbadri Elamin Osman
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引用次数: 0

Abstract

Objective: To investigate the antioxidant and hepatoprotective effects of ethanolic Mangifera indica (M. indica) seed extract on carbon tetrachloride (CCl4)-induced hepatotoxicity in albino rats.

Methods: Forty-eight albino rats weighing (100-150 g) were used for hepatoprotective and toxicity experiments. Antioxidant activity was determined using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The toxicity of M. indica seeds on the liver was evaluated by examining wellness parameters, body weight, and liver histological sections. The protective effects of 50 mg/kg and 100 mg/kg of seed extract on CCl4-induced hepatotoxicity were investigated by evaluating hematological, renal, and liver function parameters, body weight, and liver histological sections.

Results: The antioxidant activity of the M. indica ethanolic extract was (92 ± 0.03 RSA %) compared with (91 ± 0.01 RSA %) of propyl gallate, and the IC50 was (8.3 ± 0.01 µg/ml) and (14.1 ± 0.01 µg/ml). No changes were observed in the health indicators, body weights, and liver histological sections following oral administration of 50 mg/kg and 100 mg/kg of M. indica seed extracts. Treatment with M. indica seed extract significantly reduced alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), blood sugar, and urea levels compared with those in the CCl4-treated group.

Conclusion: The IC50 of the M. indica ethanolic extract was 8.3 µg/ml, and the M. indica extract is a potential source of natural antioxidants that protect against CCl4-induced hepatotoxicity.

芒果乙醇提取物通过抗氧化能力保护白化大鼠免受四氯化碳诱发的肝毒性损伤
目的研究乙醇莽草籽提取物对四氯化碳(CCl4)诱导的白化大鼠肝毒性的抗氧化和保肝作用:方法:用 48 只体重为 100-150 克的白化大鼠进行肝脏保护和毒性实验。抗氧化活性采用 2,2-二苯基-1-苦基肼(DPPH)法测定。通过检测健康参数、体重和肝脏组织切片,评估了籼稻种子对肝脏的毒性。通过评估血液学、肾脏和肝功能参数、体重和肝组织切片,研究了 50 毫克/千克和 100 毫克/千克种子提取物对 CCl4 诱导的肝毒性的保护作用:籼稻乙醇提取物的抗氧化活性为(92 ± 0.03 RSA %),没食子酸丙酯的抗氧化活性为(91 ± 0.01 RSA %),IC50 为(8.3 ± 0.01 µg/ml)和(14.1 ± 0.01 µg/ml)。口服 50 毫克/千克和 100 毫克/千克糙米茄种子提取物后,健康指标、体重和肝脏组织切片均未发生变化。与 CCl4 处理组相比,M. indica 种子提取物能显著降低丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、血糖和尿素水平:M.indica乙醇提取物的IC50值为8.3 µg/ml,M.indica提取物是一种潜在的天然抗氧化剂来源,可防止CCl4诱导的肝毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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