Improved Liver Intravital Microscopic Imaging Using a Film-Assisted Stabilization Method.

IF 8.2 1区化学 Q1 CHEMISTRY, ANALYTICAL

ACS Sensors Pub Date : 2024-10-25 Epub Date: 2024-09-03 DOI:10.1021/acssensors.4c01464

Libang Xu, Xiaobing Feng, Dazhi Wang, Fang Gao, Chenxu Feng, Qiji Shan, Ge Wang, Fang Yang, Junfeng Zhang, Jingwei Hou, Donglei Sun, Tiesheng Wang

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引用次数: 0

Abstract

Intravital microscopy (IVM) is a valuable method for biomedical characterization of dynamic processes, which has been applied to many fields such as neuroscience, oncology, and immunology. During IVM, vibration suppression is a major challenge due to the inevitable respiration and heartbeat from live animals. In this study, taking liver IVM as an example, we have unraveled the vibration inhibition effect of liquid bridges by studying the friction characteristics of a moist surface on the mouse liver. We confirmed the presence of liquid bridges on the liver through fluorescence imaging, which can provide microscale and nondestructive liquid connections between adjacent surfaces. Liquid bridges were constructed to sufficiently stabilize the liver after abdominal dissection by covering it with a polymer film, taking advantage of the high adhesion properties of liquid bridges. We further prototyped a microscope-integrated vibration-damping device with adjustable film tension to simplify the sample preparation procedure, which remarkably decreased the liver vibration. In practical application scenarios, we observed the process of liposome phagocytosis by liver Kupffer cells with significantly improved image and video quality. Collectively, our method not only provided a feasible solution to vibration suppression in the field of IVM, but also has the potential to be applied to vibration damping of precision instruments or other fields that require nondestructive ″soft″ vibration damping.

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利用胶片辅助稳定法改进肝脏内视显微成像。

内视显微镜（IVM）是一种对动态过程进行生物医学表征的重要方法，已被应用于神经科学、肿瘤学和免疫学等多个领域。在 IVM 过程中，由于活体动物不可避免地会有呼吸和心跳，因此振动抑制是一项重大挑战。本研究以肝脏 IVM 为例，通过研究小鼠肝脏潮湿表面的摩擦特性，揭示了液桥的振动抑制作用。我们通过荧光成像确认了肝脏上液体桥的存在，荧光成像可以提供相邻表面之间微观和无损的液体连接。利用液桥的高粘附性，我们在腹部解剖后在肝脏上覆盖了一层聚合物薄膜，从而构建了液桥，充分稳定了肝脏。为了简化样本制备过程，我们还进一步制作了一个可调节薄膜张力的显微镜一体化减振装置原型，该装置显著降低了肝脏的振动。在实际应用场景中，我们观察了肝脏 Kupffer 细胞吞噬脂质体的过程，图像和视频质量得到显著改善。总之，我们的方法不仅为 IVM 领域的振动抑制提供了可行的解决方案，而且有望应用于精密仪器的减振或其他需要无损″软″减振的领域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.