Drought Priming Promotes Nitrogen Use Efficiency in Wheat (Triticum aestivum L.) Under Drought Stress During Grain Filling

Abstract

Drought stress significantly challenges wheat production globally, and drought priming has emerged as an effective strategy to mitigate yield loss under drought stress events. However, the effect of drought priming on plant nitrogen use efficiency (NUE) remains insufficiently understood. This study investigates the effects of drought priming at the six-leaf stage on NUE under drought stress during grain filling in four wheat varieties with distinct responses to drought priming. Our results indicate that there is no correlation between inherent drought tolerance and the effects of drought priming among the wheat varieties studied. In priming-sensitive varieties, drought-primed plants exhibited significant improvements in grain yield and NUE under drought stress compared to non-primed plants. Conversely, priming-insensitive varieties showed no significant differences in yield or NUE between primed and non-primed plants under similar conditions. Notably, under drought stress, primed plants exhibited higher yield and NUE than non-primed plants in drought priming positive variety, while the opposite trend was observed in drought priming negative response variety. The enhancement of NUE through drought priming was associated with improved nitrogen uptake efficiency and its allocation to spikes, with abscisic acid accumulation playing a pivotal role. These findings provide valuable insights into the mechanisms by which drought priming enhances NUE under drought stress, contributing to the development of sustainable agricultural practices amid climate change.