LC.MS-based chemical profiling of Aristotle's lantern and viscera of the sea urchin Echinometra mathaei collected from the Red Sea and evaluation of their antiviral activity

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

Future Journal of Pharmaceutical Sciences Pub Date : 2024-09-02 DOI:10.1186/s43094-024-00687-1

Yousra A. Abdelaziz, Iman S. A. Khallaf, Abdallah Alian, Ahmed A. A. Ibrahim, Ezz-Eldin K. Desoky, Fahd M. Abdelkarem

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Abstract

Background

Echinometra mathaei (family Echinometridae), is one of the sea urchins widely distributed on the Egyptian coasts in the Red Sea. This organism contains edible and non-edible parts. The present study was carried out to analyze and identify the metabolites present in the non-edible parts (Aristotle's lantern and viscera) using LC/MS. Also, the cytotoxic activity on Vero cell line and antiviral activity against herpes simplex virus type 1 were evaluated using MTT colorimetric assay.

Results

Chemical profiling of the crude extracts of Aristotle's lantern and viscera using LC/MS indicated the presence of 51 and 59 compounds, respectively. The main metabolites present in both non-edible parts were phospholipids, amino acids, peptides, fatty acids and glycerol derivatives. However, the characteristic difference was the presence of carotenoid pigments only in viscera. The crude extract of Aristotle's lantern and viscera showed no cytotoxic activity on Vero cell line and significant antiviral activity against herpes simplex virus with an IC₅₀ value equal to 115.48 ± 1.20 and 122.4 ± 0.50 µg/mL, respectively.

Conclusions

In the present study, the crude extracts of the non-edible parts of E. mathaei were analyzed using LC.MS.MS.QTOF and indicated the existence of 110 chemical compounds, with significant antiviral activity against HSV-1 and no cytotoxic activity. The diversity of the identified compounds with two main categories of compounds, phospholipids and peptides, may contribute to the antiviral activity of Aristotle's lantern and viscera. Additionally, this research focused on clarification of nutritive, pharmaceutical and economic values of these parts. As future prospects, further studies are required to isolate the metabolites and assess the detailed mechanism of antiviral activity via in vitro, in vivo and in silico studies.

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基于液相色谱-质谱的红海亚里士多德灯笼和海胆内脏化学成分分析及其抗病毒活性评估

背景Echinometra mathaei（棘目海胆科）是广泛分布于埃及红海沿岸的海胆之一。该生物含有可食用和非食用部分。本研究采用 LC/MS 分析和鉴定非食用部分（亚里士多德灯笼和内脏）中的代谢物。此外，还使用 MTT 比色法评估了对 Vero 细胞系的细胞毒性活性和对 1 型单纯疱疹病毒的抗病毒活性。这两种非食用部分的主要代谢物是磷脂、氨基酸、肽、脂肪酸和甘油衍生物。不过，两者的区别在于内脏中只含有类胡萝卜素色素。亚里士多德灯笼和内脏的粗提取物对 Vero 细胞系无细胞毒性活性，但对单纯疱疹病毒有显著的抗病毒活性，IC50 值分别为 115.48 ± 1.20 和 122.4 ± 0.50 µg/mL。结论在本研究中，使用 LC.MS.MS.QTOF 分析了 E. mathaei 非食用部分的粗提取物，结果表明其中存在 110 种化学物质，对 HSV-1 具有显著的抗病毒活性，且无细胞毒性活性。鉴定出的化合物具有多样性，主要分为磷脂和肽两大类，这可能是亚里士多德灯笼草和内脏具有抗病毒活性的原因。此外，这项研究还重点阐明了这些部位的营养、医药和经济价值。展望未来，需要进一步研究分离代谢物，并通过体外、体内和硅学研究评估抗病毒活性的详细机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.