Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector

IF 3.4 3区环境科学与生态学 Q3 CELL & TISSUE ENGINEERING

Regenerative Therapy Pub Date : 2024-06-01 DOI:10.1016/j.reth.2024.08.012

Kazuto Kimura , Masaya Tsukamoto , Hiroko Sugisaki , Miyuu Tanaka , Mitsuru Kuwamura , Yuki Matsumoto , Genki Ishihara , Kei Watanabe , Mika Okada , Mahito Nakanishi , Kikuya Sugiura , Shingo Hatoya

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Abstract

Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research has not been reported. Here, we generated footprint-free fiPSCs derived from embryonic cells, as well as juvenile feline uterus-derived cells using Sendai virus vector harboring six feline-specific pluripotency-associated genes. The fiPSCs were confirmed to be of high quality with the potential to form teratomas including all three germ layers. Furthermore, our fiPSCs were maintained under feeder-free and chemically-defined conditions using StemFit® AK02N and recombinant laminin 511, iMatrix-511. Further research on fiPSCs could result in their widespread application in veterinary regenerative medicine, which could pave the way for their use in advanced regenerative medicine research for humans.

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利用仙台病毒载体生成无足迹、高质量的猫诱导多能干细胞

猫科动物和犬科动物等伴侣动物可以为从兽医到人类医学的转化研究提供一个绝佳的平台。然而，在基础或临床研究中使用优质猫科诱导多能干细胞（fiPSCs）的情况尚未见报道。在这里，我们利用仙台病毒载体（携带六个猫科动物特异的多能性相关基因）从胚胎细胞和幼猫子宫衍生细胞中生成了无足迹的fiPSCs。经证实，fiPSCs质量很高，有可能形成包括所有三个胚层在内的畸胎瘤。此外，我们的 fiPSCs 是在无喂养器和化学定义的条件下使用 StemFit® AK02N 和重组层粘连蛋白 511 iMatrix-511 保持的。对fiPSCs的进一步研究可使其在兽医再生医学中得到广泛应用，从而为其在人类先进再生医学研究中的应用铺平道路。

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来源期刊

Regenerative Therapy Engineering-Biomedical Engineering

CiteScore

6.00

自引率

2.30%

发文量

106

审稿时长

49 days

期刊介绍： Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.