GhJUB1_3-At positively regulate drought and salt stress tolerance under control of GhHB7, GhRAP2-3 and GhRAV1 in Cotton.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Adeel Ahmad, Muhammad Sajjad, Salisu Bello Sadau, Mohammad Elasad, Lu Sun, Yuewei Quan, Aimin Wu, Lian Boying, Fei Wei, Hongmei Wu, Pengyun Chen, Xiaokang Fu, Liang Ma, Hantao Wang, Hengling Wei, Shuxun Yu
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Abstract

Climate change severely affects crop production. Cotton is one of the primary fiber crops in the world and its production is susceptible to various environmental stresses, especially drought and salinity. Development of stress tolerant genotypes is the only way to escape from these environmental constraints. We identified sixteen homologs of the Arabidopsis JUB1 gene in cotton. Expression of GhJUB1_3-At was significantly induced in the temporal expression analysis of GhJUB1 genes in the roots of drought tolerant (H177) and susceptible (S9612) cotton genotypes under drought. The silencing of the GhJUB1_3-At gene alone and together with its paralogue GhJUB1_3-Dt reduced the drought tolerance in cotton plants. The transgenic lines exhibited tolerance to the drought and salt stress as compared to the wildtype (WT). The chlorophyll and relative water contents of wildtype decreased under drought as compared to the transgenic lines. The transgenic lines showed decreased H2O2 and increased proline levels under drought and salt stress, as compared to the WT, indicating that the transgenic lines have drought and salt stress tolerance. The expression analysis of the transgenic lines and WT revealed that GAI was upregulated in the transgenic lines in normal conditions as compared to the WT. Under drought and salt treatment, RAB18 and RD29A were strongly upregulated in the transgenic lines as compared to the WT. Conclusively, GhJUB1_3-At is not an auto activator and it is regulated by the crosstalk of GhHB7, GhRAP2-3 and GhRAV1. GhRAV1, a negative regulator of abiotic stress tolerance and positive regulator of leaf senescence, suppresses the expression of GhJUB1_3-At under severe circumstances leading to plant death.

在 GhHB7、GhRAP2-3 和 GhRAV1 的调控下,GhJUB1_3-At 可正向调节棉花的干旱和盐胁迫耐受性。
气候变化严重影响作物生产。棉花是世界上最主要的纤维作物之一,其生产容易受到各种环境压力的影响,尤其是干旱和盐渍化。开发抗逆基因型是摆脱这些环境限制的唯一途径。我们在棉花中发现了 16 个拟南芥 JUB1 基因的同源物。在对耐旱(H177)和易感(S9612)棉花基因型根中 GhJUB1 基因的时序表达分析中,GhJUB1_3-At 的表达被显著诱导。GhJUB1_3-At 基因单独或与其旁系亲属 GhJUB1_3-Dt 基因一起沉默都会降低棉花植株的耐旱性。与野生型(WT)相比,转基因品系表现出对干旱和盐胁迫的耐受性。与转基因品系相比,野生型在干旱条件下叶绿素和相对含水量都有所下降。与 WT 相比,转基因品系在干旱和盐胁迫下的 H2O2 水平降低,脯氨酸水平升高,这表明转基因品系具有抗旱和抗盐胁迫的能力。对转基因株系和 WT 的表达分析表明,与 WT 相比,转基因株系在正常条件下 GAI 上调。在干旱和盐胁迫条件下,与 WT 相比,RAB18 和 RD29A 在转基因品系中强烈上调。结论是,GhJUB1_3-At 并不是一个自动激活因子,它是由 GhHB7、GhRAP2-3 和 GhRAV1 共同调控的。GhRAV1 是非生物胁迫耐受性的负调控因子和叶片衰老的正调控因子,在严重情况下会抑制 GhJUB1_3-At 的表达,导致植物死亡。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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