Theileria orientalis Ikeda infection detected in red deer but not dogs or horses in New Zealand.

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES
K E Lawrence, K Gedye, L Carvalho, B Wang, L M Fermin, W E Pomroy
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引用次数: 0

Abstract

Aims: To determine whether evidence for infection with Theileria orientalis (Ikeda) could be identified in samples of commercial red deer (Cervus elaphus), horses, and working farm dogs in New Zealand.

Methods: Blood samples were collected during October and November 2019 from a convenience sample of red deer (n = 57) at slaughter. Equine blood samples (n = 50) were convenience-sampled from those submitted to a veterinary pathology laboratory for routine testing in January 2020. Blood samples, collected for a previous study from a convenience sample of Huntaway dogs (n = 115) from rural regions throughout the North and South Islands of New Zealand between August 2018 and December 2020, were also tested. DNA was extracted and quantitative PCR was used to detect the T. orientalis Ikeda major piroplasm surface protein (MPSP) gene. A standard curve of five serial 10-fold dilutions of a plasmid carrying a fragment of the T. orientalis MPSP gene was used to quantify the number of T. orientalis organisms in the samples. MPSP amplicons obtained by end-point PCR on positive samples were isolated and subjected to DNA sequencing. The resulting sequences were compared to previously published T. orientalis sequences.

Results: There were 6/57 (10%) samples positive for T. orientalis Ikeda from the deer and no samples positive for T. orientalis Ikeda from the working dogs or horses. The mean infection intensity for the six PCR-positive deer was 5.1 (min 2.2, max 12.4) T. orientalis Ikeda organisms/µL.

Conclusions and clinical relevance: Red deer can potentially sustain low infection intensities of T. orientalis Ikeda and could act as reservoirs of infected ticks. Further studies are needed to determine whether naïve ticks feeding on infected red deer can themselves become infected.

Abbreviations: Cq: Quantification cycle; LOQ: Limits of quantification; MPSP: Major piroplasm surface protein; qPCR: Quantitative polymerase chain reaction.

在新西兰发现东方马尾线虫病感染者,但没有发现狗或马。
目的:确定能否在新西兰的商品赤鹿(Cervus elaphus)、马和农场工作犬样本中发现感染东方马尾线虫病(Theileria orientalis (Ikeda))的证据:方法:2019 年 10 月和 11 月期间,从屠宰时的红鹿样本(n = 57)中采集了血液样本。马的血液样本(n = 50)是从 2020 年 1 月提交给兽医病理实验室进行常规检测的马的血液样本中方便抽取的。此外,还检测了 2018 年 8 月至 2020 年 12 月期间从新西兰南北岛农村地区方便抽取的亨特威犬(n = 115)血液样本。提取 DNA 并使用定量 PCR 检测 T. orientalis Ikeda 主要螺旋体表面蛋白(MPSP)基因。用 5 个连续 10 倍稀释的携带有东方蓟马 MPSP 基因片段的质粒的标准曲线来量化样本中东方蓟马的数量。在阳性样本上通过终点 PCR 获得的 MPSP 扩增子被分离出来并进行 DNA 测序。结果显示,6/57(10%)的样本中发现了东方尺蠖:结果:6/57(10%)份鹿样本对池田东革热菌呈阳性反应,而工作犬和马的样本对池田东革热菌均无阳性反应。6 只 PCR 阳性鹿的平均感染强度为 5.1(最低 2.2,最高 12.4)个池田东方蝶菌/微升:红鹿有可能维持较低的池田东方蜱感染强度,并可能成为受感染蜱虫的储库。还需要进一步研究来确定以受感染的赤鹿为食的天真蜱本身是否会受到感染:缩写:Cq:缩写:Cq:定量周期;LOQ:定量限;MPSP:主要螺浆表面蛋白;qPCR:定量聚合酶链反应:定量聚合酶链反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
New Zealand veterinary journal
New Zealand veterinary journal 农林科学-兽医学
CiteScore
3.00
自引率
0.00%
发文量
37
审稿时长
12-24 weeks
期刊介绍: The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health. The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally. Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife. All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.
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