Use of Zebrafish (Danio rerio) for Biosafety Evaluation of Strontium Nanostructured Hydroxyapatite

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Elisângela da Costa Silva, Augusto Monteiro de Souza, Alexandre Malta Rossi, Andrea Machado Costa, José Mauro Grangeiro, Ana Carolina Luchiari, Silvia Regina Batistuzzo de Medeiros
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Abstract

Despite the numerous studies on biocompatibility with nano-biomaterials, the biological effects of strontium-substituted HA nanoparticles (nSrHA) need to be better understood. So, we conducted an embryotoxicity test using zebrafish (Danio rerio) according to the OECD 236 guideline, a model that represents a viable alternative that bridges the gap between in vitro and mammalian models. Zebrafish embryos were exposed for 120 h to microspheres containing nSrHA nanoparticles with low and high crystallinity, synthesized at temperatures of 5°C (nSrHA5) and 90°C (nSrHA90). We evaluated lethality, developmental parameters, and reactive oxygen species (ROS) production. The larval behavior was assessed at 168 hpf to determine if the biomaterials affected motor responses and anxiety-like behavior. The results showed that the survival rate decreased significantly for the nSrHA5 group (low crystalline particles), and an increase in ROS was also observed in this group. However, none of the biomaterials caused morphological changes indicative of toxicity during larval development. Additionally, the behavioral tests did not reveal any alterations in all experimental groups, indicating the absence of neurotoxic effects from exposure to the tested biomaterials. These findings provide valuable insights into the biosafety of modified HA-based nanostructured biomaterials, making them a promising strategy for bone tissue repair. As the use of hydroxyapatite-based biomaterials continues to grow, it is crucial to ensure rigorous control over the quality, reliability, and traceability of these materials.

Abstract Image

利用斑马鱼(Danio rerio)评估锶纳米结构羟基磷灰石的生物安全性。
尽管对纳米生物材料的生物相容性进行了大量研究,但锶取代的 HA 纳米粒子(nSrHA)的生物效应仍有待进一步了解。因此,我们根据 OECD 236 准则,使用斑马鱼(Danio rerio)进行了胚胎毒性试验。将斑马鱼胚胎暴露在含有低结晶度和高结晶度 nSrHA 纳米颗粒的微球中 120 小时,这些微球是在 5°C (nSrHA5) 和 90°C (nSrHA90) 温度下合成的。我们评估了致死率、发育参数和活性氧(ROS)产生情况。在168 hpf时对幼虫行为进行评估,以确定生物材料是否会影响运动反应和焦虑行为。结果表明,nSrHA5 组(低结晶颗粒)的存活率明显下降,同时还观察到该组的 ROS 增加。不过,在幼虫发育过程中,所有生物材料都没有引起显示毒性的形态变化。此外,所有实验组的行为测试均未发现任何变化,这表明接触受测生物材料不会产生神经毒性效应。这些发现为改性 HA 纳米结构生物材料的生物安全性提供了宝贵的见解,使其成为一种很有前景的骨组织修复策略。随着羟基磷灰石基生物材料的使用不断增加,确保对这些材料的质量、可靠性和可追溯性进行严格控制至关重要。
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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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