Development and Characterization of a Novel Composite Hydrogel Biomaterial for Improved Mucoperiosteal Wound Repair

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Q. Wang, M. Dunnwald, D. S. F. Kacmarynski, K. S. Worthington
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Abstract

Mucoperiosteal wound healing, as it occurs after pediatric cleft palate surgery, can be challenging due to the limitations of current treatments such as tissue flaps secured with sutures and fibrin glue. In this study, we characterized the in vitro performance of a novel composite hydrogel biomaterial designed to be employed as an in situ wound filler and enhance mucoperiosteal wound healing. We evaluated a range of photopolymerizable formulations containing methacrylated gelatin (GelMA), glycol chitosan, and bioglass microparticles. Our aim was to identify one or more formulations with an appropriate balance of properties against a set of functional requirements that we established for this application. To test the formulations against these criteria, we measured photopolymerization kinetics, mechanical properties, degradation rate, in vitro biocompatibility, and ex vivo tissue adhesion. All formulations polymerized in less than 90 s using violet light. In addition, we found that GelMA-based hydrogels were more adhesive to mucoperiosteal tissue than clinical standard fibrin glue. Inclusion of small amounts of bioglass in the formulation increased mechanical compatibility with mucoperiosteal tissue, enhanced cytoconductivity, and promoted cell proliferation. Taken together, our results support the suitability of these photopolymerized composite hydrogels as in situ mucoperiosteal wound fillers. Overall, this study lays the groundwork for investigating the in vivo, pre-clinical effectiveness of these composite hydrogels in improving mucoperiosteal wound healing outcomes.

Abstract Image

开发新型复合水凝胶生物材料并确定其特性,以改善粘骨膜伤口修复。
小儿腭裂手术后会出现粘骨膜伤口愈合,由于目前的治疗方法(如用缝线和纤维蛋白胶固定组织瓣)存在局限性,因此粘骨膜伤口愈合具有挑战性。在本研究中,我们对一种新型复合水凝胶生物材料的体外性能进行了表征,这种材料可用作原位伤口填充物,并能促进粘骨膜伤口愈合。我们评估了一系列含有甲基丙烯酸明胶(GelMA)、乙二醇壳聚糖和生物玻璃微粒的光聚合配方。我们的目的是找出一种或多种配方,这些配方的特性与我们为该应用制定的一系列功能要求之间达到了适当的平衡。为了根据这些标准对配方进行测试,我们测量了光聚合动力学、机械性能、降解率、体外生物相容性和体内外组织粘附性。使用紫光时,所有配方都能在 90 秒内完成聚合。此外,我们还发现,与临床标准纤维蛋白胶相比,基于 GelMA 的水凝胶对粘骨膜组织的粘附性更强。在配方中加入少量生物玻璃可提高与粘骨膜组织的机械相容性,增强细胞传导性,促进细胞增殖。综上所述,我们的研究结果支持将这些光聚合复合水凝胶用作原位粘骨膜伤口填充物。总之,这项研究为研究这些复合水凝胶在改善粘骨膜伤口愈合效果方面的体内临床前有效性奠定了基础。
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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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