Reclassification of Butyrivibrio crossotus Moore et al. 1976 (Approved Lists 1980) into a novel genus as Eshraghiella crossota gen. nov., comb. nov.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-01 DOI:10.1099/ijsem.0.006509

Mehdi Fatahi-Bafghi

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Abstract

The reclassification of Butyrivibrio crossotus Moore et al. 1976 (Approved Lists 1980) as Eshraghiella crossota gen. nov., comb. nov. is proposed within the family Lachnospiraceae. This reclassification is based on differences revealed through the analysis of 16S rRNA, groEL, recA, and rpoB genes, as well as genome sequences, distinguishing it from other Butyrivibrio species. Comparative analysis showed that B. crossotus exhibited digital DNA-DNA hybridization (dDDH) values of 19.40-27.20% and average nucleotide identities based on blast (ANIb) values of 67.06-67.64% with other Butyrivibrio species. These values are significantly below the species delineation thresholds (dDDH, 70%; ANIb, 95-96%), justifying the proposed reclassification. Additionally, the results of the average amino acid identity (AAI) analysis indicated that this species shares 59.22-60.17% AAI with the other species of the genus Butyrivibrio, which is below the AAI threshold (65%) for a genus boundary. In addition, biochemical and morphological characteristics also support the proposal that this species is different from other species of the genus Butyrivibrio. The type strain is ATCC 29175^T (DSM 2876^T=T9-40A^T).

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将 Butyrivibrio crossotus Moore 等人 1976 年的分类（1980 年批准清单）改为新属，即 Eshraghiella crossota gen.

建议将 Butyrivibrio crossotus Moore 等人 1976 年（1980 年批准清单）重新分类为 Eshraghiella crossota gen.这一重新分类的依据是通过分析 16S rRNA、groEL、recA 和 rpoB 基因以及基因组序列所发现的差异，将其与其他布氏痢疾杆菌物种区分开来。比较分析表明，交叉嗜血杆菌与其他布氏梭菌的数字 DNA-DNA 杂交（dDDH）值为 19.40-27.20%，基于 blast 的平均核苷酸相同度（ANIb）值为 67.06-67.64%。这些值明显低于物种划分阈值（dDDH，70%；ANIb，95-96%），因此建议重新分类。此外，平均氨基酸同一性（AAI）分析结果表明，该物种与布氏痢疾杆菌属的其他物种共享 59.22-60.17% 的 AAI，低于属界的 AAI 临界值（65%）。此外，生化和形态特征也支持该物种不同于布氏弧菌属其他物种的观点。模式菌株为 ATCC 29175T（DSM 2876T=T9-40AT）。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464