Reshaped commensal wound microbiome via topical application of Calvatia gigantea extract contributes to faster diabetic wound healing.

IF 6.3 1区医学 Q1 DERMATOLOGY

Burns & Trauma Pub Date : 2024-09-02 eCollection Date: 2024-01-01 DOI:10.1093/burnst/tkae037

Xiaotong Ding, Chenxi Yang, Yue Li, Tangtang He, Yan Xu, Xuxi Cheng, Jinyun Song, Nannan Xue, Wen Min, Weimeng Feng, Hongyu Zhao, Jie Dong, Pei Liu, Yiwei Wang, Jun Chen

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Abstract

Background: Calvatia gigantea (CG) is widely used as a traditional Chinese medicine for wound treatment. In this study, we aimed to determine the effects of CG extract (CGE) on diabetic wound healing and the commensal wound microbiome.

Method: A wound model was established using leptin receptor-deficient db/db mice, with untreated mice as the control group and CGE-treated mice as the treatment group. The wound healing rate, inflammation and histology were analyzed. Additionally, wound microbiome was evaluated via 16S ribosomal RNA (rRNA) gene sequencing.

Results: CGE significantly accelerated the healing of diabetic ulcer wounds, facilitated re-epithelialization, and downregulated the transcription levels of the inflammatory cytokines, interleukin-1β and tumor necrosis factor-α. Furthermore, CGE treatment positively affected the wound microbiome, promoting diversity of the microbial community and enrichment of Escherichia-Shigella bacteria in the CGE-treated group.

Conclusions: Overall, CGE enhanced diabetic wound healing by modulating the wound microbiome and facilitating macrophage polarization during inflammation. These findings suggest modulation of the commensal wound microbiome using medicinal plants as a potential therapeutic strategy for diabetic wounds.

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通过局部应用钙华菊提取物重塑共生伤口微生物群有助于加快糖尿病伤口愈合。

背景：石菖蒲（CG）被广泛用作治疗伤口的传统中药。本研究旨在确定石杉碱甲提取物（CGE）对糖尿病伤口愈合和伤口微生物群的影响：方法：利用瘦素受体缺陷的 db/db 小鼠建立伤口模型，以未处理的小鼠为对照组，CGE 处理的小鼠为治疗组。对伤口愈合率、炎症和组织学进行分析。此外，还通过 16S 核糖体 RNA（rRNA）基因测序评估了伤口微生物组：结果：CGE能明显加速糖尿病溃疡伤口的愈合，促进伤口的再上皮化，并能降低炎症细胞因子、白细胞介素-1β和肿瘤坏死因子-α的转录水平。此外，CGE 还对伤口微生物组产生了积极影响，促进了微生物群落的多样性，并富集了 CGE 治疗组中的埃希氏-志贺氏菌：总之，CGE 通过调节伤口微生物群和促进炎症期间巨噬细胞的极化，促进了糖尿病伤口的愈合。这些研究结果表明，利用药用植物调节共生伤口微生物群是治疗糖尿病伤口的一种潜在策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Burns & Trauma 医学-皮肤病学

CiteScore

8.40

自引率

9.40%

发文量

186

审稿时长

6 weeks

期刊介绍： The first open access journal in the field of burns and trauma injury in the Asia-Pacific region, Burns & Trauma publishes the latest developments in basic, clinical and translational research in the field. With a special focus on prevention, clinical treatment and basic research, the journal welcomes submissions in various aspects of biomaterials, tissue engineering, stem cells, critical care, immunobiology, skin transplantation, and the prevention and regeneration of burns and trauma injuries. With an expert Editorial Board and a team of dedicated scientific editors, the journal enjoys a large readership and is supported by Southwest Hospital, which covers authors'' article processing charges.