Bacillus subtilis grown in a "breathing" vessel without sparger aeration.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-22 DOI:10.1093/bbb/zbae120

Ken-Ichi Yoshida, Kyosuke Yokoyama, Ayşegül Öktem, Shu Ishikawa, Jan Maarten van Dijl, Masato Yotsuya, Ryosuke Sato

引用次数: 0

Abstract

Here we present a "breathing" vessel consisting of expanded polytetrafluoroethylene, which allows gas exchange but no liquid permeation. The bacterial culture inside needs only agitation to promote air supply. Using this setup, a Bacillus subtilis cell factory for scyllo-inositol production grew to produce scyllo-inositol efficiently. The results indicate that our approach represents a sustainable "greener" approach for the cell factory.

查看原文本刊更多论文

枯草芽孢杆菌在 "会呼吸 "的容器中生长，不使用喷射器通气。

在这里，我们介绍一种由膨化聚四氟乙烯组成的 "呼吸 "容器，它允许气体交换，但不允许液体渗透。内部的细菌培养只需搅拌即可促进空气供应。利用这种装置，用于生产丝胶肌醇的枯草芽孢杆菌细胞工厂得以生长，并高效地生产出丝胶肌醇。结果表明，我们的方法是一种可持续的 "绿色 "细胞工厂方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.