Regional Analysis of the Immune Microenvironment in Human Endometrium

IF 2.5 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2024-09-03 DOI:10.1111/aji.13921

Lingtao Yang, Yiyi Su, Songchen Cai, Huan Ma, Jing Yang, Mingjuan Xu, Yuye Li, Chunyu Huang, Yong Zeng, Qiyuan Li, Mingqian Feng, Hanjie Li, Lianghui Diao

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Abstract

Problem

Endometrial immune cells are essential for maintaining homeostasis and the endometrial receptivity to embryo implantation. Understanding regional variations in endometrial immune cell populations is crucial for comprehending normal endometrial function and the pathophysiology of endometrial disorders. Despite previous studies focusing on the overall immune cell composition and function in the endometrium, regional variations in premenopausal women remain unclear.

Method of Study

Endometrial biopsies were obtained from four regions (anterior, posterior, left lateral, and right lateral) of premenopausal women undergoing hysteroscopy with no abnormalities. A 15-color human endometrial immune cell-focused flow cytometry panel was used for analysis. High-dimensional flow cytometry combined with a clustering algorithm was employed to unravel the complexity of endometrial immune cells. Additionally, multiplex immunofluorescent was performed for further validation.

Results

Our findings revealed no significant variation in the distribution and abundance of immune cells across different regions under normal conditions during the proliferative phase. Each region harbored similar immune cell subtypes, indicating a consistent immune microenvironment. However, when comparing normal regions to areas with focal hemorrhage, significant differences were observed. An increase in CD8⁺ T cells highlights the impact of localized abnormalities on the immune microenvironment.

Conclusions

Our study demonstrates that the endometrial immune cell landscape is consistent across different anatomical regions during the proliferative phase in premenopausal women. This finding has important implications for understanding normal endometrial function and the pathophysiology of endometrial disorders.

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人类子宫内膜免疫微环境的区域分析

问题：子宫内膜免疫细胞对维持子宫内膜的平衡和胚胎着床至关重要。了解子宫内膜免疫细胞群的区域性变化对于理解子宫内膜的正常功能和子宫内膜疾病的病理生理学至关重要。尽管以前的研究侧重于子宫内膜免疫细胞的整体组成和功能，但绝经前妇女的区域性变化仍不清楚：研究方法：从接受宫腔镜检查且无异常的绝经前妇女的四个区域（前方、后方、左外侧和右外侧）获取子宫内膜活检组织。采用 15 色人类子宫内膜免疫细胞聚焦流式细胞术面板进行分析。高维流式细胞术与聚类算法相结合，揭示了子宫内膜免疫细胞的复杂性。此外，还进行了多重免疫荧光进一步验证：结果：我们的研究结果表明，在增殖期的正常情况下，不同区域免疫细胞的分布和丰度无明显差异。每个区域都有相似的免疫细胞亚型，这表明免疫微环境是一致的。然而，当将正常区域与局灶性出血区域进行比较时，观察到了显著的差异。CD8+ T细胞的增加凸显了局部异常对免疫微环境的影响：我们的研究表明，在绝经前妇女的增殖期，不同解剖区域的子宫内膜免疫细胞格局是一致的。这一发现对了解正常子宫内膜功能和子宫内膜疾病的病理生理学具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.