Visualization of Biomolecular Radiation Damage at the Single-Particle Level Using Lanthanide-Sensitized DNA Origami.

IF 11.3 1区化学 Q1 CHEMISTRY, PHYSICAL

ACS Catalysis Pub Date : 2024-09-18 Epub Date: 2024-09-03 DOI:10.1021/acs.nanolett.4c03307

Minle Chen, Yijuan Jiang, Yongjie Zhang, Xiaoling Chen, Lei Xie, Lili Xie, Tao Zeng, Yana Liu, Hao Liu, Min Wang, Xiaofeng Chen, Zhenzhen Zhang, Yu He, Xian Qin, Chunhua Lu, Qiushui Chen, Huanghao Yang

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引用次数: 0

Abstract

Precise monitoring of biomolecular radiation damage is crucial for understanding X-ray-induced cell injury and improving the accuracy of clinical radiotherapy. We present the design and performance of lanthanide-DNA-origami nanodosimeters for directly visualizing radiation damage at the single-particle level. Lanthanide ions (Tb³⁺ or Eu³⁺) coordinated with DNA origami nanosensors enhance the sensitivity of X-ray irradiation. Atomic force microscopy (AFM) revealed morphological changes in Eu³⁺-sensitized DNA origami upon X-ray irradiation, indicating damage caused by ionization-generated electrons and free radicals. We further demonstrated the practical applicability of Eu³⁺-DNA-origami integrated chips in precisely monitoring radiation-mediated cancer radiotherapy. Quantitative results showed consistent trends with flow cytometry and histological examination under comparable X-ray irradiation doses, providing an affordable and user-friendly visualization tool for preclinical applications. These findings provide new insights into the impact of heavy metals on radiation-induced biomolecular damage and pave the way for future research in developing nanoscale radiation sensors for precise clinical radiography.

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利用镧系元素敏化 DNA 折纸在单粒子水平实现生物分子辐射损伤的可视化。

精确监测生物分子辐射损伤对于了解 X 射线诱导的细胞损伤和提高临床放疗的准确性至关重要。我们介绍了用于在单粒子水平直接观察辐射损伤的镧系元素-DNA原型纳米计量器的设计和性能。与 DNA 折纸纳米传感器配位的镧系离子（Tb3+ 或 Eu3+）提高了 X 射线辐照的灵敏度。原子力显微镜（AFM）显示，Eu3+敏化的DNA折纸在X射线辐照下发生了形态变化，表明电离产生的电子和自由基造成了破坏。我们进一步证明了 Eu3+-DNA-origami 集成芯片在精确监测辐射介导的癌症放疗中的实用性。定量结果显示，在可比的 X 射线照射剂量下，流式细胞术和组织学检查的趋势一致，为临床前应用提供了一种经济实惠、用户友好的可视化工具。这些发现为重金属对辐射诱导的生物分子损伤的影响提供了新的见解，并为未来开发用于精确临床放射摄影的纳米级辐射传感器的研究铺平了道路。

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来源期刊

ACS Catalysis CHEMISTRY, PHYSICAL-

CiteScore

20.80

自引率

6.20%

发文量

1253

审稿时长

1.5 months

期刊介绍： ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels. The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.