MYB immunohistochemistry as a predictor of MYB::NFIB fusion in the diagnosis of adenoid cystic carcinoma of the head and neck

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2024-08-16 DOI:10.1016/j.oooo.2024.08.006

Thomas J.W. Klein Nulent MD, DMD , Robert J.J. van Es MD, DMD, PhD , Gerben E. Breimer MD, PhD , Matthijs H. Valstar MD, DMD , Laura A. Smit MD, PhD , Caroline M. Speksnijder MSc, PhD , Remco de Bree MD, PhD , Stefan M. Willems MD, PhD

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引用次数: 0

Abstract

Objectives

Diagnosing adenoid cystic carcinoma (AdCC) is challenging due to histopathological variability and similarities with other tumors. In AdCC pathogenesis, the cellular myeloblastosis gene (c-MYB) often exhibits a MYB::NFIB fusion from a reciprocal translocation. This study aimed to assess the predictive accuracy of MYB immunohistochemistry for detecting this translocation compared to fluorescence in situ hybridization (FISH).

Study design

This study included 110 AdCC patients (1999-2017) from two Dutch head and neck centers using tissue microarrays and full slides. Median MYB expression levels by immunohistochemistry were compared based on translocation status by FISH, and differences within clinicopathological parameters were examined. An immunohistochemical cut-off was established to estimate the translocation.

Results

MYB immunohistochemistry was available in 90/110 patients, with a median expression of 27%. FISH was interpretable in 79/108 tumors, identifying MYB::NFIB fusion in 44 (56%). Among 62 patients with both MYB expression and translocation data, the fusion was present in 38 (61%). These tumors had higher MYB expression (30%) than nontranslocated tumors (6%); P = .02. A 60% MYB expression cut-off yielded 100% specificity for detecting the translocation but had no prognostic value.

Conclusions

Although MYB protein expression alone lacks diagnostic precision, protein expression >60% predicted the MYB::NFIB fusion in all tumors.

查看原文本刊更多论文

在头颈部腺样囊性癌诊断中，MYB 免疫组化可预测 MYB::NFIB 融合。

目的：腺样囊性癌（AdCC）的组织病理学变异性和与其他肿瘤的相似性使诊断具有挑战性。在腺样囊性癌的发病机制中，细胞成髓细胞增多症基因（c-MYB）通常表现为MYB::NFIB融合的互易易位。本研究旨在评估与荧光原位杂交（FISH）相比，MYB免疫组化检测这种易位的预测准确性：本研究使用组织芯片和完整切片，纳入了来自荷兰两家头颈部中心的110例AdCC患者（1999-2017年）。根据FISH的易位状态比较免疫组化的中位MYB表达水平，并检查临床病理参数的差异。结果显示：MYB免疫组化表达水平的中位数与FISH检测的易位状态进行了比较，并考察了临床病理参数之间的差异：结果：90/110 例患者进行了 MYB 免疫组化，中位表达率为 27%。79/108例肿瘤可进行FISH解读，其中44例（56%）确定了MYB::NFIB融合。在 62 例同时具有 MYB 表达和易位数据的患者中，有 38 例（61%）存在融合。这些肿瘤的 MYB 表达量（30%）高于非易位肿瘤（6%）；P = 0.02。60%的MYB表达临界值对检测易位有100%的特异性，但没有预后价值：尽管单独检测MYB蛋白表达缺乏诊断准确性，但蛋白表达>60%可预测所有肿瘤中的MYB::NFIB融合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.