Abscisic acid-induced H2O2 production positively regulates the activity of SAPK8/9/10 through oxidation of the type one protein phosphatase OsPP47

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-09-01 DOI:10.1111/nph.20092

Caihua Qin, Xing Fan, Qianqian Fang, Honghua Yu, Lan Ni, Mingyi Jiang

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引用次数: 0

Abstract

Subclass III sucrose nonfermenting1-related protein kinase 2s (SnRK2s) are positive regulators of abscisic acid (ABA) signaling and abiotic stress responses. However, the underlying activation mechanisms of osmotic stress/ABA-activated protein kinase 8/9/10 (SAPK8/9/10) of rice (Oryza sativa) subclass III SnRK2s in ABA signaling remain to be elucidated.
In this study, we employed biochemical, molecular biology, cell biology, and genetic approaches to identify the molecular mechanism by which OsPP47, a type one protein phosphatase in rice, regulates SAPK8/9/10 activity in ABA signaling.
We found that OsPP47 not only physically interacted with SAPK8/9/10 but also interacted with ABA receptors PYLs. OsPP47 negatively regulated ABA sensitivity in seed germination and root growth. In the absence of ABA, OsPP47 directly inactivated SAPK8/9/10 by dephosphorylation. In the presence of ABA, ABA-bound OsPYL2 formed complexes with OsPP47 and inhibited its phosphatase activity, partially releasing the inhibition of SAPK8/9/10. SAPK8/9/10-mediated H₂O₂ production inhibited OsPP47 activity by oxidizing Cys-116 and Cys-256 to form OsPP47 oligomers, resulting in not only preventing the OsPP47-SAPK8/9/10 interaction but also blocking the inhibition of SAPK8/9/10 activity by OsPP47.
Our results reveal novel pathways for the inhibition of SAPK8/9/10 in the basal state and for the activation of SAPK8/9/10 induced by ABA in rice.

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脱落酸诱导产生的 H2O2 通过氧化 1 型蛋白磷酸酶 OsPP47 来正向调节 SAPK8/9/10 的活性。

亚类III蔗糖不发酵1相关蛋白激酶2（SnRK2s）是脱落酸（ABA）信号传导和非生物胁迫反应的正调控因子。然而，水稻（Oryza sativa）III亚类SnRK2s的渗透胁迫/ABA激活蛋白激酶8/9/10（SAPK8/9/10）在ABA信号转导中的潜在激活机制仍有待阐明。在本研究中，我们采用生物化学、分子生物学、细胞生物学和遗传学方法，确定了水稻中的一种蛋白磷酸酶 OsPP47 在 ABA 信号转导中调控 SAPK8/9/10 活性的分子机制。我们发现，OsPP47不仅与SAPK8/9/10有物理作用，还与ABA受体PYLs有相互作用。OsPP47 负向调节种子萌发和根系生长中的 ABA 敏感性。在没有 ABA 的情况下，OsPP47 通过去磷酸化直接使 SAPK8/9/10 失活。在存在 ABA 的情况下，与 ABA 结合的 OsPYL2 与 OsPP47 形成复合物并抑制其磷酸酶活性，从而部分解除对 SAPK8/9/10 的抑制。SAPK8/9/10 介导的 H2O2 生成通过氧化 Cys-116 和 Cys-256 形成 OsPP47 寡聚体来抑制 OsPP47 的活性，结果不仅阻止了 OsPP47-SAPK8/9/10 的相互作用，而且阻断了 OsPP47 对 SAPK8/9/10 活性的抑制。我们的研究结果揭示了水稻在基础状态下抑制 SAPK8/9/10 以及在 ABA 诱导下激活 SAPK8/9/10 的新途径。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.