{"title":"Role of fimbriae variations in Porphyromonas gulae biofilm formation","authors":"Sho Yoshida , Hiroaki Inaba , Ryota Nomura , Kazuhiko Nakano , Michiyo Matsumoto-Nakano","doi":"10.1016/j.job.2024.08.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div><em>Porphyromonas gulae</em> is a major causative agent of periodontal disease in companion animals that possesses various virulence factors, including fimbriae, lipopolysaccharides, and proteases. <em>P. gulae</em> fimbriae are classified into three genotypes (A, B, and C) based on their nucleotide sequences. Type C fimbrial isolates have been reported to be more virulent than other <em>fimA</em> types, suggesting that different <em>fimA</em> types may aid in the regulation of periodontal pathogenesis. Detailed findings regarding the ability of <em>P. gulae</em> to form biofilms have yet to be reported. Here, we investigated the contributions of fimbrial genotypes in <em>P. gulae</em> biofilm formation.</div></div><div><h3>Methods</h3><div><em>P. gulae</em> and <em>P. gingivalis</em> biofilms were generated on plates and analyzed using confocal laser microscopy. Additionally, the biofilms formed were assessed by staining with crystal violet. Furthermore, the physical strength of <em>P. gulae</em> biofilms was examined by ultrasonication.</div></div><div><h3>Results</h3><div>Biofilms formed by <em>P. gulae</em> type C were denser than those formed by types A and B. Moreover, the amount of biofilm formed by type C strains was significantly greater than that formed by type A and B strains, which was similar to the biofilms formed by <em>P. gingivalis</em> with type II fimbriae. Additionally, the physical strength of the type C biofilm was significantly greater than that of the other strains.</div></div><div><h3>Conclusions</h3><div>These results suggest that FimA variation may coordinate for biofilm formation. This is the first report on the observation and characterization of <em>P. gulae</em> biofilm formation.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 4","pages":"Pages 28-33"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1349007924001932","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives
Porphyromonas gulae is a major causative agent of periodontal disease in companion animals that possesses various virulence factors, including fimbriae, lipopolysaccharides, and proteases. P. gulae fimbriae are classified into three genotypes (A, B, and C) based on their nucleotide sequences. Type C fimbrial isolates have been reported to be more virulent than other fimA types, suggesting that different fimA types may aid in the regulation of periodontal pathogenesis. Detailed findings regarding the ability of P. gulae to form biofilms have yet to be reported. Here, we investigated the contributions of fimbrial genotypes in P. gulae biofilm formation.
Methods
P. gulae and P. gingivalis biofilms were generated on plates and analyzed using confocal laser microscopy. Additionally, the biofilms formed were assessed by staining with crystal violet. Furthermore, the physical strength of P. gulae biofilms was examined by ultrasonication.
Results
Biofilms formed by P. gulae type C were denser than those formed by types A and B. Moreover, the amount of biofilm formed by type C strains was significantly greater than that formed by type A and B strains, which was similar to the biofilms formed by P. gingivalis with type II fimbriae. Additionally, the physical strength of the type C biofilm was significantly greater than that of the other strains.
Conclusions
These results suggest that FimA variation may coordinate for biofilm formation. This is the first report on the observation and characterization of P. gulae biofilm formation.
目的:古拉卟啉单胞菌是伴侣动物牙周病的主要致病菌,具有多种毒力因子,包括缘膜、脂多糖和蛋白酶。根据核苷酸序列,P. gulae 菌膜可分为三种基因型(A、B 和 C)。据报道,C型缘核膜分离物比其他fimA型的毒性更强,这表明不同的fimA型可能有助于调节牙周致病机理。有关 P. gulae 形成生物膜能力的详细研究结果尚未见报道。在此,我们研究了fimbrial基因型在P. gulae生物膜形成中的贡献:方法:在平板上生成 P. gulae 和 P. gingivalis 生物膜,并使用激光共聚焦显微镜进行分析。此外,还通过水晶紫染色法对形成的生物膜进行了评估。此外,还用超声波检测了 P. gulae 生物膜的物理强度:此外,C 型菌株形成的生物膜数量明显多于 A 型和 B 型菌株形成的生物膜,这与带有 II 型指状体的牙龈球菌形成的生物膜相似。此外,C 型生物膜的物理强度明显高于其他菌株:这些结果表明,FimA 的变异可能会协调生物膜的形成。这是第一份关于古拉痢疾杆菌生物膜形成的观察和特征描述的报告。