Kevin Rodriguez, Lloyd Kao, Vincent E Cerbantez-Bueno, Christian Delgadillo, Dorothy Nguyen, Samin Ullah, Cameron Delgadillo, G Venugopala Reddy
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引用次数: 0
Abstract
The precise regulation of stem cells in the shoot apical meristems (SAMs) involves the function of the homeodomain transcription factor (TF)-WUSCHEL (WUS). WUS has been shown to move from the site of production-the rib-meristem (RM), into overlaying cells of the central zone (CZ), where it specifies stem cells and also regulates the transcription of CLAVATA3 (CLV3). The secreted signalling peptide CLV3 activates a receptor kinase signalling that restricts WUS transcription and also regulates the nuclear gradient of WUS by offsetting nuclear export. WUS has been shown to regulate both CLV3 levels and spatial activation, restricting its expression to a few cells in the CZ. The HAIRY MERISTEM (HAM), a GRASS-domain class of TFs expressed in the RM, has been shown to physically interact with WUS and regulate CLV3 expression. However, the mechanisms by which this interaction regulates CLV3 expression non-cell autonomously remain unclear. Here, we show that HAM function is required for regulating the WUS protein stability, and the CLV3 expression responds to altered WUS protein levels in ham mutants. Thus, HAM proteins non-cell autonomously regulates CLV3 expression.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.