HAIRY MERISTEM proteins regulate the WUSCHEL protein levels in mediating CLAVATA3 expression.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Kevin Rodriguez, Lloyd Kao, Vincent E Cerbantez-Bueno, Christian Delgadillo, Dorothy Nguyen, Samin Ullah, Cameron Delgadillo, G Venugopala Reddy
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引用次数: 0

Abstract

The precise regulation of stem cells in the shoot apical meristems (SAMs) involves the function of the homeodomain transcription factor (TF)-WUSCHEL (WUS). WUS has been shown to move from the site of production-the rib-meristem (RM), into overlaying cells of the central zone (CZ), where it specifies stem cells and also regulates the transcription of CLAVATA3 (CLV3). The secreted signalling peptide CLV3 activates a receptor kinase signalling that restricts WUS transcription and also regulates the nuclear gradient of WUS by offsetting nuclear export. WUS has been shown to regulate both CLV3 levels and spatial activation, restricting its expression to a few cells in the CZ. The HAIRY MERISTEM (HAM), a GRASS-domain class of TFs expressed in the RM, has been shown to physically interact with WUS and regulate CLV3 expression. However, the mechanisms by which this interaction regulates CLV3 expression non-cell autonomously remain unclear. Here, we show that HAM function is required for regulating the WUS protein stability, and the CLV3 expression responds to altered WUS protein levels in ham mutants. Thus, HAM proteins non-cell autonomously regulates CLV3 expression.

HAIRY MERISTEM 蛋白在介导 CLAVATA3 表达的过程中调节 WUSCHEL 蛋白水平。
对嫩枝顶端分生组织(SAM)中干细胞的精确调控涉及同源转录因子(TF)-WUSCHEL(WUS)的功能。研究表明,WUS会从产生部位--肋-分生组织(RM)移动到中央区(CZ)的重叠细胞中,在那里指定干细胞,并调节CLAVATA3(CLV3)的转录。分泌的信号肽CLV3会激活受体激酶信号,从而限制WUS的转录,并通过抵消核输出来调节WUS的核梯度。研究表明,WUS 可调节 CLV3 的水平和空间激活,将其表达限制在 CZ 中的少数细胞内。HAIRY MERISTEM(HAM)是在RM中表达的一类GRASS-domain TFs,已被证明能与WUS发生物理相互作用并调节CLV3的表达。然而,这种相互作用非细胞自主调节 CLV3 表达的机制仍不清楚。在这里,我们发现 HAM 的功能是调节 WUS 蛋白稳定性所必需的,而且在 ham 突变体中,CLV3 的表达会对 WUS 蛋白水平的改变做出反应。因此,HAM蛋白非细胞自主调节CLV3的表达。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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