Elucidation of AsANS controlling pigment biosynthesis in Angelica sinensis through hormonal and transcriptomic analysis.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Khadija Tehseen Arshad, Chunfan Xiang, Chengxiao Yuan, Lesong Li, Juan Wang, Pinhan Zhou, Nazer Manzoor, Shengchao Yang, Mengfei Li, Yanli Liang, Junwen Chen, Yan Zhao
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Abstract

Angelica sinensis, a traditional Chinese medicinal plant, has been primarily reported due to its nutritional value. Pigmentation in this plant is an important appearance trait that directly affects its commercial value. To understand the mechanism controlling purpleness in A. sinensis, hormonal and transcriptomic analyses were performed in three different tissues (leave, root and stem), using two cultivars with contrasting colors. The two-dimensional data set provides dynamic hormonal and gene expression networks underpinning purpleness in A. sinensis. We found abscisic acid as a crucial hormone modulating anthocyanin biosynthesis in A. sinensis. We further identified and validated 7 key genes involved in the anthocyanin biosynthesis pathway and found a specific module containing ANS as a hub gene in WGCNA. Overexpression of a candidate pigment regulatory gene, AsANS (AS08G02092), in transgenic calli of A. sinensis resulted in increased anthocyanin production and caused purpleness. Together, these analyses provide an important understanding of the molecular networks underlying A. sinensis anthocyanin production and its correlation with plant hormones, which can provide an important source for breeding.

通过激素和转录组分析阐明控制当归色素生物合成的 AsANS。
当归是一种传统的中药植物,主要因其营养价值而被报道。这种植物的色素沉着是一种重要的外观性状,直接影响其商业价值。为了了解控制当归色素纯度的机制,研究人员利用两个颜色对比强烈的栽培品种,在三个不同组织(叶、根和茎)中进行了激素和转录组分析。二维数据集提供了支持中华皂荚变紫的动态激素和基因表达网络。我们发现脱落酸是调节中华秋海棠花青素生物合成的关键激素。我们进一步鉴定并验证了参与花青素生物合成途径的 7 个关键基因,并在 WGCNA 中发现了一个包含 ANS 的特定模块,该模块是一个枢纽基因。在转基因中华榕树胼胝体中过表达候选色素调控基因 AsANS (AS08G02092),可增加花青素的产生并使其变白。总之,这些分析为了解中华鳖花青素产生的分子网络及其与植物激素的相关性提供了重要信息,为育种提供了重要依据。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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