Exploring the antimicrobial potential of crude peptide extracts from Allium sativum and Allium oschaninii against antibiotic-resistant bacterial strains.

IF 3.9 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Pharmaceutical Biology Pub Date : 2024-12-01 Epub Date: 2024-08-28 DOI:10.1080/13880209.2024.2395517
Thitiluck Swangsri, Onrapak Reamtong, Sompob Saralamba, Pakavadee Rakthong, Urusa Thaenkham, Naowarat Saralamba
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引用次数: 0

Abstract

Context: Plant peptides garner attention for their potential antimicrobial properties amid the rising concern over antibiotic-resistant bacteria.

Objective: This study investigates the antibacterial potential of crude peptide extracts from 27 Thai plants collected locally.

Materials and methods: Peptide extracts from 34 plant parts, derived from 27 Thai plants, were tested for their antimicrobial efficacy against four highly resistant bacterial strains: Streptococcus aureus MRSA, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli. The stability of these peptide extracts was examined at different temperatures, and the synergistic effects of two selected plant peptide extracts were investigated. Additionally, the time-kill kinetics of the individual extracts and their combination were determined against the tested pathogens.

Results: Peptides from Allium sativum L. and Allium oschaninii O. Fedtsch (Amaryllidaceae) were particularly potent, inhibiting bacterial growth with MICs ranging from 1.43 to 86.50 µg/mL. The consistent MICs and MBCs of these extracts across various extraction time points highlight their reliability. Stability tests reveal that these peptides maintain their antimicrobial activity at -20 °C for over a month, emphasizing their durability for future exploration and potential applications in addressing antibiotic resistance. Time-kill assays elucidate the time and concentration-dependent nature of these antimicrobial effects, underscoring their potent initial activity and sustained efficacy over time.

Discussion and conclusions: This study highlights the antimicrobial potential of Allium-derived peptides, endorsing them for combating antibiotic resistance and prompting further investigation into their mechanisms.

探索薤白和薤白肽粗提取物对抗生素耐药菌株的抗菌潜力。
背景:在抗生素耐药菌问题日益受到关注的背景下,植物肽因其潜在的抗菌特性而备受关注:本研究调查了从泰国当地收集的 27 种植物中提取的粗肽的抗菌潜力:从 27 种泰国植物中提取的 34 种植物部位的肽提取物对四种高度耐药细菌菌株进行了抗菌效果测试:这些细菌包括:金黄色葡萄球菌 MRSA、铜绿假单胞菌、鲍曼不动杆菌和大肠杆菌。研究了这些肽提取物在不同温度下的稳定性,并探讨了两种选定植物肽提取物的协同作用。此外,还测定了单个提取物及其组合对受试病原体的时间杀伤动力学:结果:来自 Allium sativum L. 和 Allium oschaninii O. Fedtsch(Amaryllidaceae)的多肽特别有效,其抑制细菌生长的 MIC 为 1.43 至 86.50 µg/mL。在不同的提取时间点,这些提取物的 MIC 和 MBC 一致,这凸显了它们的可靠性。稳定性测试表明,这些肽能在零下 20 摄氏度的环境中保持一个月以上的抗菌活性,强调了它们在未来探索中的耐久性以及在解决抗生素耐药性方面的潜在应用。时间杀伤试验阐明了这些抗菌效果的时间和浓度依赖性,强调了它们强大的初始活性和长期的持续功效:本研究强调了薤白肽的抗菌潜力,支持它们用于抗击抗生素耐药性,并促使人们进一步研究它们的作用机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pharmaceutical Biology
Pharmaceutical Biology 医学-药学
CiteScore
6.70
自引率
2.60%
发文量
191
审稿时长
1 months
期刊介绍: Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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