Characterization of the conversion system of natural rubber to poly(3-Hydroxyalkanoate) in Piscinibacter gummiphilus strain NS21T

IF 4.5 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

New biotechnology Pub Date : 2024-08-30 DOI:10.1016/j.nbt.2024.08.507

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Abstract

Poly(3-hydroxyalkanoate) (PHA), a bacteria-synthesized biodegradable polyester, is a useful alternative to fossil resources, and current systems for its production rely predominantly on edible resources, raising concerns about microbial competition for nutrients. Therefore, we investigated mechanisms underlying PHA production from non-edible resources by Piscinibacter gummiphilus strain NS21^T. Strain NS21^T can utilize natural rubber as a carbon source on solid media and potentially produces PHA. Gas chromatography and nuclear magnetic resonance analyses of NS21^T cell extracts revealed the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(3-hydroxybutyrate) from natural rubber and glucose, respectively. Transcriptional analysis suggested that phaC is involved in PHA production. An increased PHBV accumulation rate under nitrogen-limiting conditions indicates the potential of this strain to be used as a PHBV production enhancement strategy. Furthermore, the disruption of PHA depolymerase genes resulted in enhanced PHA production, indicating the involvement of these genes in PHA degradation. These findings highlight the potential of NS21^T for PHBV production from natural rubber, a non-edible resource.

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gummiphilus Piscinibacter 菌株 NS21T 中天然橡胶到聚（3-羟基烷酸）转化系统的表征。

聚（3-羟基烷酸）（PHA）是一种由细菌合成的可生物降解的聚酯，是化石资源的有用替代品，目前的生产系统主要依赖于可食用资源，这引起了人们对微生物争夺营养物质的担忧。因此，我们研究了 gummiphilus 鱼腥藻菌株 NS21T 利用非食用资源生产 PHA 的机制。菌株 NS21T 可在固体培养基上利用天然橡胶作为碳源，并有可能产生 PHA。对 NS21T 细胞提取物的气相色谱和核磁共振分析表明，该菌株可分别从天然橡胶和葡萄糖中生产聚（3-羟基丁酸-3-羟基戊酸）（PHBV）和聚（3-羟基丁酸）。转录分析表明 phaC 参与了 PHA 的生产。在氮限制条件下，PHBV 的积累率增加，这表明该菌株有潜力用作 PHBV 生产的增强策略。此外，PHA 解聚酶基因的破坏导致 PHA 产量提高，表明这些基因参与了 PHA 降解。这些发现凸显了 NS21T 从天然橡胶（一种非食用资源）中生产 PHBV 的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New biotechnology 生物-生化研究方法

CiteScore

11.40

自引率

1.90%

发文量

审稿时长

1 months

期刊介绍： New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.

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