Genotype selection identified elite lines through quantitative trait loci mapping of agronomically important traits in wheat.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-08-31 eCollection Date: 2024-09-01 DOI:10.1007/s11032-024-01496-3

Yunlong Pang, Liming Wang, Linzhi Li, Xiaoqian Wang, Danfeng Wang, Meng Zhao, Chenhao Ma, Huirui Zhang, Qiang Yan, Yue Lu, Yunlong Liang, Xiangsheng Kong, Huaqiang Zhu, Xuecheng Sun, Yujie Zhao, Shubing Liu

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Abstract

Wheat is one of the most important staple foods in the world. Genetic characterization of wheat agronomically important traits is crucial for yield improvement through molecular breeding. In this study, a recombinant inbred line (RIL) population was developed by crossing a local adapted high yield variety Jimai 22 (JM22) with an external variety Cunmai no.1 (CM1). A high-density genetic map containing 7,359 single nucleotide polymorphism (SNP) markers was constructed. Quantitative trait loci (QTL) mapping identified 61 QTL for eight yield-related traits under six environments (years). Among them, 17 QTL affecting spike number per plant, grain number per spike and thousand grain weight showed high predictability for theoretical yield per plant (TYP), of which, 12 QTL alleles positively contributed to TYP. Nine promising candidate genes for seven of the 12 QTL were identified including three known wheat genes and six rice orthologs. Four elite lines with TYP increased by 5.6%-15.2% were identified through genotype selection which carried 7-9 favorable alleles from JM22 and 2-3 favorable alleles from CM1 of the 12 QTL. Moreover, the linked SNPs of the 12 QTL were converted to high-throughput kompetitive allele-specific PCR (KASP) markers and validated in the population. The mapped QTL, identified promising candidate genes, developed elite lines and KASP markers are highly valuable in future genotype selection to improve wheat yield.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01496-3.

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通过绘制小麦重要农艺性状的数量性状位点图，进行基因型选择，从而鉴定出优良品系。

小麦是世界上最重要的主食之一。小麦重要农艺性状的遗传特征对于通过分子育种提高产量至关重要。本研究通过本地适应性高产品种吉麦 22（JM22）与外来品种存麦 1 号（CM1）杂交，培育了一个重组近交系（RIL）群体。构建了包含 7,359 个单核苷酸多态性（SNP）标记的高密度遗传图谱。定量性状位点（QTL）图谱确定了六种环境（年）下八个产量相关性状的 61 个 QTL。其中，影响单株穗数、单株穗粒数和千粒重的 17 个 QTL 对理论单株产量（TYP）有较高的预测性，其中 12 个 QTL 等位基因对 TYP 有正贡献。在 12 个 QTL 中，为其中 7 个 QTL 确定了 9 个有希望的候选基因，包括 3 个已知的小麦基因和 6 个水稻直向同源基因。通过基因型选择，确定了 4 个 TYP 提高 5.6%-15.2% 的精英品系，它们携带 12 个 QTL 中 7-9 个来自 JM22 的有利等位基因和 2-3 个来自 CM1 的有利等位基因。此外，12个QTL的连锁SNP被转化为高通量等位基因特异性PCR（KASP）标记，并在群体中进行了验证。绘制的 QTL 图谱、确定的有希望的候选基因、开发的精英品系和 KASP 标记对未来提高小麦产量的基因型选择极具价值：在线版本包含补充材料，可查阅 10.1007/s11032-024-01496-3。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.