Molecular analysis of cutaneous yeast isolates in the mycobiota of children with atopic dermatitis.

IF 2.7 3区医学 Q3 INFECTIOUS DISEASES

Medical mycology Pub Date : 2024-09-06 DOI:10.1093/mmy/myae090

Iwyna França Souza Gomes Vial, Morgana Ferreira Voidaleski, Rosângela Ferreira Lameira, Flavia de Fatima Costa, Danielle Arake Zanatta, Vania Aparecida Vicente, Vania Oliveira de Carvalho

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Abstract

The skin of patients with atopic dermatitis (AD) has a greater diversity of mycobiota. An observational, prospective, cross-sectional, analytical, and comparative study was conducted involving 80 patients with AD Group (ADG) and 50 individuals without AD (wADG) in a tertiary hospital in Brazil. Skin scale samples were collected from the frontal, cervical, fossae cubital, and popliteal regions and identified using molecular biology techniques. The results showed that 47.5% of ADG had identified yeasts compared to 0% of wADG (P < .001). The yeasts Rhodotorula mucilaginosa and Candida parapsilosis were the most abundant. The probability of colonization increased with age, showing values of 40% at 60 months and 80% at 220 months (P = .09). The cervical region (12.5%) was colonized to the greatest extent. Our findings revealed that positive mycology was not more probable when the scoring of atopic dermatitis or eczema area and severity index value increased (P = .23 and .53, respectively). The results showed that the sex, age, and different population types directly affected the composition of the mycobiota in the population analyzed. A higher frequency of colonization and greater diversity of yeast species were detected in the cutaneous mycobiota of children with AD.

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特应性皮炎患儿真菌生物群中皮肤酵母分离物的分子分析。

特应性皮炎（AD）患者皮肤上的真菌生物群更加多样化。巴西一家三甲医院对 80 名特应性皮炎患者（ADG）和 50 名非特应性皮炎患者（wADG）进行了一项观察性、前瞻性、横断面分析和比较研究。研究人员从额头、颈部、肘窝和腘窝部位采集了皮肤鳞片样本，并使用分子生物学技术进行了鉴定。结果显示，47.5% 的 ADG 发现了酵母菌，而 0% 的 wADG 发现了酵母菌（p < 0.001）。酵母菌中以粘液酵母菌和副酵母菌最多。随着年龄的增长，定植的概率也在增加，60 个月时为 40%，220 个月时为 80%（p = 0.09）。宫颈部位（12.5%）的定植率最高。我们的研究结果表明，当 SCORAD 或 EASI 值增加时，霉菌学阳性的可能性并不大（p = 0.23 和 0.53）。结果表明，性别、年龄和不同的人群类型直接影响了所分析人群中霉菌生物群的组成。在AD患儿的皮肤真菌生物群中发现了更高的定植频率和更多的酵母菌种类。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical mycology 医学-兽医学

CiteScore

5.70

自引率

3.40%

发文量

632

审稿时长

12 months

期刊介绍： Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.