Reliability of In Vivo Creatine-Weighted Chemical Exchange Saturation Transfer (CrCEST) MRI in Calf Skeletal Muscle of Healthy Volunteers at 3 T.

IF 3.3 2区 医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Kasturee Chakraborty, Ritambhar Burman, Sabah Nisar, Saorla Miller, Zachary Loschinskey, Shengjie Wu, Yimei Li, Asim K Bag, Ayaz Khan, Chelsea Goodenough, Neil Wilson, Mohammad Haris, Shana E McCormack, Ravinder Reddy, Kirsten Ness, Richard Finkel, Puneet Bagga
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引用次数: 0

Abstract

Background: Skeletal muscle mitochondrial oxidative phosphorylation (mtOXPHOS) is important for ATP generation and its dysfunction leads to exercise intolerance. Phosphorus magnetic resonance spectroscopy (31P-MRS) is a useful, noninvasive technique for mtOXPHOS assessment but has limitations. Creatine-weighted chemical exchange saturation transfer (CrCEST) MRI is a potential alternative to assess muscle bioenergetics.

Purpose: To evaluate the interscan repeatability, intra- and interobserver reproducibility of CrCEST during mild plantar flexion exercise.

Study type: Retrospective.

Subjects: Twenty healthy volunteers (age 37.6 ± 12.4 years, 11 females).

Field strength/sequence: 3 T/CEST imaging using gradient echo readout.

Assessment: τCrCEST (postexercise Cr recovery time) was assessed in two scans for each participant, following mild plantar flexion exercises targeting the medial gastrocnemius (MG), lateral gastrocnemius (LG), and soleus (Sol) muscles. Three observers measured τCrCEST for interobserver reproducibility. Three readings by one observer were used to measure intraobserver reproducibility. Two scans were used for within-participant interscan repeatability.

Statistical tests: Paired t tests, intraclass correlation coefficient (ICC), and Pearson correlation were conducted. Bland-Altman plots were used to analyze the interobserver variability. A P-value of 0.05 was considered statistically significant.

Results: There was excellent intra- (ICC 0.94 - 0.98 $$ \in \left[0.94-0.98\right] $$ ) and interobserver (ICC 0.9 - 0.98 $$ \in \left[0.9-0.98\right] $$ ) reproducibility, with moderate interscan repeatability for τCrCEST in LG and MG (ICC 0.54 - 0.74 $$ \in \left[0.54-0.74\right] $$ ) and poor-to-moderate interscan repeatability in Sol (ICC 0.24 - 0.53 $$ \in \left[0.24-0.53\right] $$ ). Excellent interobserver reproducibility was confirmed by Bland-Altman plots (fixed bias P-value 0.08 - 0.87 $$ \in \left[0.08-0.87\right] $$ ).

Data conclusion: CrCEST MRI shows promise in assessing muscle bioenergetics by evaluating τCrCEST during mild plantar flexion exercise with reasonable reliability, particularly in LG and MG.

Level of evidence: 4 TECHNICAL EFFICACY: Stage 1.

3 T 下健康志愿者小腿骨骼肌体内肌酸加权化学交换饱和转移 (CrCEST) MRI 的可靠性。
背景:骨骼肌线粒体氧化磷酸化(mtOXPHOS)对 ATP 的生成非常重要,其功能障碍会导致运动不耐受。磷磁共振波谱(31P-MRS)是评估 mtOXPHOS 的一种有用、无创技术,但也有其局限性。目的:评估轻度跖屈运动中 CrCEST 的扫描间可重复性、观察者内部和观察者之间的可重复性:研究类型:回顾性:20 名健康志愿者(年龄 37.6 ± 12.4 岁,11 名女性):评估:在针对内侧腓肠肌(MG)、外侧腓肠肌(LG)和比目鱼肌(Sol)进行轻度跖屈运动后,对每位受试者的τCrCEST(运动后Cr恢复时间)进行两次扫描评估。三名观察者测量了 τCrCEST 以确保观察者之间的重复性。一名观察者的三次读数用于测量观察者内部的再现性。两次扫描用于测量参与者内部扫描间的可重复性:进行了配对 t 检验、类内相关系数 (ICC) 和皮尔逊相关性检验。使用 Bland-Altman 图分析观察者间的变异性。P 值为 0.05 即为具有统计学意义:结果:在LG和MG中,τCrCEST有极好的内部(ICC ∈ 0.94 - 0.98 $$ \ in \left[0.94-0.98\right] $$)和观察者间(ICC ∈ 0.9 - 0.98 $$ \ in \left[0.9-0.98\right] $$)可重复性,扫描间可重复性中等(ICC ∈ 0.54 - 0.74 $$ \ in \left[0.54-0.74\right] $$),而在Sol(ICC∈ 0.24 - 0.53 $$ \ in \left[0.24-0.53\right] $$)的扫描间重复性为差到中等。Bland-Altman图证实了极好的观察者间可重复性(固定偏倚P值∈ 0.08 - 0.87 $$ (in \left[0.08-0.87\right]$$):数据结论:CrCEST MRI通过评估轻度跖屈运动中的τCrCEST,显示了评估肌肉生物能的前景,尤其是在LG和MG中,具有合理的可靠性:4 技术功效:第一阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.70
自引率
6.80%
发文量
494
审稿时长
2 months
期刊介绍: The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.
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