Cloning and expression profiling of peptide YY in the brain of common carp, Cyprinus carpio

IF 2.1 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

General and comparative endocrinology Pub Date : 2024-08-30 DOI:10.1016/j.ygcen.2024.114600

Cheni-Chery Sudhakumari , Dharavath Bhasker , Sonika Kar, Akanksha Pranoty, Aparna Dutta-Gupta , Balasubramanian Senthilkumaran

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Abstract

Peptide YY (PYY) is an anorectic brain-gut pancreatic peptide that helps in feeding regulation by reducing appetite and is well characterized in mammals. The role of PYY in relation to brain is least studied in mammals as well as in lower vertebrates including fish, however high expression was evident in male reproductive tissue. In this regard, this study attempts to evaluate the significance of PYY in the brain of common carp, Cyprinus carpio. As a first step, the cDNA of PYY from brain of adult male carp was cloned. Following which expression analysis was performed using juvenile and adult fish. The differential distribution pattern in various regions of brain and ontogeny expression analysis indicated that PYY may involve in physiological processes related to brain-pituitary axis. In addition, a significant decrease in neuropeptide Y expression was observed upon PYY- endoribonuclease-prepared small interfering RNA transfection in brain cells, in vitro indicating plausible PYY-NPY interaction in brain-pituitary axis of common carp.

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鲤鱼脑中多肽 YY 的克隆和表达谱分析。

肽 YY（PYY）是一种厌食的脑肠胰肽，它通过降低食欲来帮助调节进食，在哺乳动物中具有很好的特征。在哺乳动物以及包括鱼类在内的低等脊椎动物中，对PYY在大脑中的作用研究得最少，但在雄性生殖组织中却有明显的高表达。因此，本研究试图评估PYY在鲤鱼大脑中的重要性。首先，亚克隆了成年雄性鲤鱼大脑中PYY的cDNA。随后，利用幼鱼和成鱼进行了表达分析。PYY在大脑各区域的不同分布模式和本体表达分析表明，PYY可能参与了与脑-垂体轴相关的生理过程。此外，体外观察到，PYY-内切核酸酶制备的小干扰 RNA 转染脑细胞后，神经肽 Y 的表达量明显下降，这表明PYY-NPY 之间可能存在相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

General and comparative endocrinology 医学-内分泌学与代谢

CiteScore

5.60

自引率

7.40%

发文量

120

审稿时长

2 months

期刊介绍： General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.