Paper-Based procalcitonin and Interleukin-6 test strip with Spectrum-Based optical reader for enterovirus severity differentiation in children

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2024-08-29 DOI:10.1016/j.cca.2024.119942

Cheng-Han Chen , Rui-Ling Huang , Ching-Chuan Liu , Chitsung Hong , Yunching Chen , Ching-Fen Shen , Chao-Min Cheng

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引用次数: 0

Abstract

Infectious diseases significantly impact global health, necessitating prompt diagnosis to mitigate life-threatening sepsis risk. Identifying patients at risk of severe neurological complications from enterovirus infections is challenging due to nonspecific initial presentations. Point-of-care testing (POCT) has emerged as a transformative tool, with low-cost lateral-flow colorimetric assays showing promise in deployable POCT devices. We developed a PCT/IL-6 rapid diagnostic system integrating lateral flow assay (LFA) test strips and a portable optical spectrum reader, allowing simultaneous semi-quantitative measurement of serum PCT and IL-6 within 30 min at the point of care. The system demonstrated a strong correlation with traditional ELISA and effectively differentiated severe pediatric enterovirus cases using serum samples. IL-6 showed superior discriminatory ability over PCT in identifying patients with severe neurological complications. This novel diagnostic platform holds great potential for early sepsis recognition and infectious disease management, especially in resource-limited settings.

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纸质降钙素原和白细胞介素-6 检测试纸条，带基于光谱的光学读取器，用于区分儿童肠道病毒的严重程度。

传染病严重影响全球健康，需要及时诊断以降低危及生命的败血症风险。由于初期表现不具特异性，识别肠道病毒感染导致严重神经系统并发症的高危患者具有挑战性。床旁检测（POCT）已成为一种变革性工具，低成本的侧流比色法检测在可部署的 POCT 设备中大有可为。我们开发了一种 PCT/IL-6 快速诊断系统，该系统集成了侧流检测（LFA）试纸条和便携式光学光谱阅读器，可在 30 分钟内在护理点同时对血清 PCT 和 IL-6 进行半定量检测。该系统与传统的酶联免疫吸附法有很强的相关性，能有效区分使用血清样本的严重小儿肠道病毒病例。在鉴别严重神经系统并发症患者方面，IL-6的鉴别能力优于PCT。这种新型诊断平台在早期败血症识别和传染病管理方面具有巨大潜力，尤其是在资源有限的环境中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.