Relaxin-2 promotes osteoblastic differentiation mediated by epidermal growth factor and epidermal growth factor receptor signaling.

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biotechnology and applied biochemistry Pub Date : 2024-09-01 DOI:10.1002/bab.2661

Lankai Yi, Ning Han, Zhong Li, Housen Jiang, Zhenhao Cao

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Abstract

Loss of osteogenic differentiation potential of osteoblasts has been associated with the pathogenesis of osteoporosis. Thus, stimulation of osteoblastic differentiation is a therapeutic strategy for osteoporosis. Relaxin-2 is a peptide hormone with potent biological functions. However, the effects of Relaxin-2 in osteoblastic differentiation and osteoporosis have not been reported before. Here, we report a novel physiological role of Relaxin-2 in promoting osteoblastic differentiation and mineralization of MC3T3-E1 cells. Our results indicate that exposure to Relaxin-2 upregulated the expression, and elevated the activity of alkaline phosphatase (ALP) when MC3T3-E1 cells were cultured in osteogenic differentiation medium (OM). Additionally, Relaxin-2 upregulated the mRNA levels of osteocalcin (ocn), osteopontin (opn), and collagen type I alpha 1 (Col1a1). The alizarin red S staining assay revealed that Relaxin-2 promoted the mineralization of MC3T3-E1 cells. We also found that Relaxin-2 increased the expression of Runx-2 as well as the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR). Importantly, silencing of EGF abolished the effects of Relaxin-2 in osteoblastic differentiation and related gene expression. These findings suggest that Relaxin-2 stimulates osteogenic differentiation through activating EGF/EGFR signaling.

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松弛素-2 通过表皮生长因子和表皮生长因子受体信号传导促进成骨细胞分化。

成骨细胞成骨分化潜能的丧失与骨质疏松症的发病机制有关。因此，刺激成骨细胞分化是治疗骨质疏松症的一种策略。松弛素-2 是一种肽类激素，具有强大的生物功能。然而，Relaxin-2 在成骨细胞分化和骨质疏松症中的作用尚未见报道。在这里，我们报告了松弛素-2在促进 MC3T3-E1 细胞成骨细胞分化和矿化中的一种新的生理作用。我们的研究结果表明，在成骨分化培养基（OM）中培养 MC3T3-E1 细胞时，暴露于 Relaxin-2 会上调碱性磷酸酶（ALP）的表达并提高其活性。此外，Relaxin-2 还能上调骨钙素（ocn）、骨生成素（opn）和Ⅰ型α1 胶原（Col1a1）的 mRNA 水平。茜素红 S 染色试验显示，Relaxin-2 促进了 MC3T3-E1 细胞的矿化。我们还发现，Relaxin-2 增加了 Runx-2 以及表皮生长因子（EGF）和表皮生长因子受体（EGFR）的表达。重要的是，沉默表皮生长因子可消除松弛素-2对成骨细胞分化和相关基因表达的影响。这些发现表明，Relaxin-2 通过激活表皮生长因子/表皮生长因子受体信号刺激成骨分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology and applied biochemistry 工程技术-生化与分子生物学

CiteScore

6.00

自引率

7.10%

发文量

117

审稿时长

3 months

期刊介绍： Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation. The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.