Organotypic culture of post-mortem adult human brain explants exhibits synaptic plasticity

IF 7.6 1区医学 Q1 CLINICAL NEUROLOGY

Brain Stimulation Pub Date : 2024-09-01 DOI:10.1016/j.brs.2024.08.010

Yukiko Iwasaki , Corentin Bernou , Barbara Gorda , Sophie Colomb , Gowrishankar Ganesh , Raphael Gaudin

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引用次数: 0

Abstract

Background

Synaptic plasticity is an essential process encoding fine-tuned brain functions, but models to study this process in adult human systems are lacking.

Objective

We aim to test whether ex vivo organotypic culture of post-mortem adult brain explants (OPABs) retain synaptic plasticity.

Methods

OPABs were seeded on 3D microelectrode arrays to measure local field potential (LFP). Paired stimulation of distant electrodes was performed over three days to investigate our capacity to modulate specific neuronal connections.

Results

Long-term potentiation (LTP) or depression (LTD) did not occur within a single day. In contrast, after two and three days of training, OPABs showed a significant modulation of the paired electrodes’ response compared to the non-paired electrodes from the same array. This response was alleviated upon treatment with dopamine.

Conclusion

Our work highlights that adult human brain explants retain synaptic plasticity, offering novel approaches to neural circuitry in animal-free models.

查看原文本刊更多论文

死后成人大脑外植体的有机培养显示出突触可塑性。

背景：突触可塑性是编码大脑微调功能的重要过程，但目前还缺乏在成人人体系统中研究这一过程的模型：/假设：我们旨在测试死后成人脑外植体（OPAB）的体外器官型培养是否保留突触可塑性：在三维微电极阵列上播种 OPAB，以测量局部场电位（LFP）。在三天内对远处电极进行配对刺激，以研究我们调节特定神经元连接的能力：结果：一天内不会出现长期电位（LTP）或抑制（LTD）。相反，经过两天和三天的训练后，与来自同一阵列的非配对电极相比，OPABs 对配对电极的反应显示出显著的调节作用。结论：（s）：我们的工作强调了成人人脑外植体保留了突触可塑性，为在无动物模型中研究神经回路提供了新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Stimulation 医学-临床神经学

CiteScore

13.10

自引率

9.10%

发文量

256

审稿时长

72 days

期刊介绍： Brain Stimulation publishes on the entire field of brain stimulation, including noninvasive and invasive techniques and technologies that alter brain function through the use of electrical, magnetic, radiowave, or focally targeted pharmacologic stimulation. Brain Stimulation aims to be the premier journal for publication of original research in the field of neuromodulation. The journal includes: a) Original articles; b) Short Communications; c) Invited and original reviews; d) Technology and methodological perspectives (reviews of new devices, description of new methods, etc.); and e) Letters to the Editor. Special issues of the journal will be considered based on scientific merit.