Reduced aggregation of the leghorn male hepatoma cell line in suspension by supplementing dextran sulfate in the media.

IF 2.4 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-02-01 Epub Date: 2024-08-27 DOI:10.5713/ab.24.0372
Jung Soo Lim, Ji Soo Kim, Yugyeong Cheon, Tae Sub Park, Jong Kwang Hong
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Abstract

Objective: The study aimed to improve the efficiency of leghorn male hepatoma (LMH) cells for animal virus vaccine production by transitioning from adherent to suspension culture and evaluating the effects of dextran sulfate (DS) on preventing cell aggregation. The goal was to enhance cell growth, viability, and glucose metabolism and to develop efficient suspension-adapted LMH cells for large-scale vaccine production.

Methods: LMH cells previously cultured in an adherent state were transferred to 125 mL Erlenmeyer flasks to conduct suspension culture. Cell culture performance, including cell density, viability, and glucose metabolism, during the cultures was measured, along with an assessment of cell aggregation. Additionally, mRNA expression levels of genes associated with cell adhesion and apoptosis were monitored.

Results: DS supplementation in suspension culture enhanced cell viability and growth, with higher cell densities and viabilities compared to control media. Additionally, DS supplementation reduced glucose consumption and waste production, indicating improved metabolic efficiency. DS also delayed cell aggregation, possibly by downregulating integrin expression and promoting anti-apoptotic gene expression. However, even after 2 months, cell aggregation persisted in both control and DS-supplemented cultures, suggesting further optimization is needed for LMH cell adaptation to suspension culture.

Conclusion: DS supplementation in LMH cell suspension cultures led to notable improvements in cell growth, viability, and glucose metabolism, while also decreasing the cell aggregation.

通过在培养基中添加硫酸葡聚糖,减少悬浮液中的莱霍恩雄性肝瘤(LMH)细胞系的聚集。
研究目的该研究旨在通过从粘附培养过渡到悬浮培养,并评估硫酸葡聚糖(DS)对防止细胞聚集的影响,从而提高用于动物病毒疫苗生产的莱霍恩雄性肝瘤(LMH)细胞的效率。目的是提高细胞的生长、活力和葡萄糖代谢,并为大规模疫苗生产开发高效的悬浮适配 LMH 细胞:方法:将先前以粘附状态培养的 LMH 细胞转移到 125 mL Erlenmeyer 烧瓶中进行悬浮培养。在培养过程中测量了细胞培养性能,包括细胞密度、存活率和葡萄糖代谢,并评估了细胞聚集情况。此外,还监测了细胞粘附和凋亡相关基因的 mRNA 表达水平:结果:与对照培养基相比,在悬浮培养中补充 DS 提高了细胞的活力和生长,细胞密度和活力更高。此外,补充 DS 还减少了葡萄糖的消耗和废物的产生,表明新陈代谢效率有所提高。DS 还可能通过下调整合素表达和促进抗凋亡基因表达来延缓细胞聚集。然而,即使在2个月后,细胞聚集在对照组和补充DS的培养物中仍然存在,这表明LMH细胞对悬浮培养的适应性需要进一步优化:结论:在 LMH 细胞悬浮培养中补充 DS 能显著改善细胞的生长、活力和葡萄糖代谢,同时还能减少细胞聚集。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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