The Role of MiR-375 in Migration and Invasion of H.pylori-induced Gastric Cancer Cell Model.

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell Biochemistry and Biophysics Pub Date : 2024-08-30 DOI:10.1007/s12013-024-01473-9

Zhichao Mao, Xinyu Wang, Yongtang Zhao, Fei Yang, Qin Qin, Ruilian Jiang

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引用次数: 0

Abstract

This article aimed to investigate the mechanism of miR-375 in Hp-induced gastric cancer cells (GCCs) model. Human normal gastric mucosal epithelial cell (GMEC) line GES-1 and human GCCs strain MKN45 were used as research objects. The expression of miR-375 was detected after H.pylori (Hp) infection of GCCs. The cell activity was detected by, 53-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and the cell multiplication was determined by cell counting kit-8 (CCK-8) method. Transwell assay was used to detect the effect of cell invasion and migration ability. The expression levels of JAK1 and STAT3 proteins were determined by Western blot (WB). MiR-375 was increased in GCCs after Hp infection, and JAK1, STAT3, p-JAK1, and p-STAT3 in GCCs after Hp infection were visibly increased. In addition, the overexpressed miR-375 promoted the multiplication activity, migration, and invasion ability of GCCs. MiR-375 promotes Hp-induced migration and invasion of GCCs by targeting JAK1/STAT3. This article reveals the important role of miR-375 in Hp-induced GC, which provides new clues for further study of its mechanism and therapeutic targets.

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MiR-375 在幽门螺杆菌诱导的胃癌细胞模型迁移和侵袭中的作用

本文旨在研究 miR-375 在 Hp 诱导的胃癌细胞（GCCs）模型中的作用机制。以人正常胃黏膜上皮细胞（GMEC）系 GES-1 和人 GCCs 株 MKN45 为研究对象。在幽门螺杆菌（Hp）感染 GCCs 后，检测了 miR-375 的表达。细胞活性用 53-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）法检测，细胞增殖用细胞计数试剂盒-8（CCK-8）法测定。Transwell 试验用于检测细胞侵袭和迁移能力的影响。通过 Western blot（WB）检测 JAK1 和 STAT3 蛋白的表达水平。Hp感染后，GCCs中的MiR-375增加，Hp感染后GCCs中的JAK1、STAT3、p-JAK1和p-STAT3明显增加。此外，过表达的miR-375还能促进GCCs的繁殖活性、迁移和侵袭能力。MiR-375通过靶向JAK1/STAT3促进Hp诱导的GCCs迁移和侵袭。本文揭示了miR-375在Hp诱导的GCC中的重要作用，为进一步研究其机制和治疗靶点提供了新的线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

7.5 months

期刊介绍： Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.