Andressa N R Leal, Winnie Q Brandão, Maurício F de Aguiar, Dionísio G Kór, Emanoel L T França, Celso P de Melo, Yeda M B de Almeida
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引用次数: 0
Abstract
We prepared zinc oxide nanoparticles (ZnO NPs) via a green synthesis and used them for the fluorescence sensing of ascorbic acid (AA). For obtaining these nanoparticles, we used an extract from Batavia lettuce as a reducing agent for zinc acetate in a simple, fast, and environmentally friendly synthesis. The ZnO NPs were characterized by X-ray diffractometry (XRD), ultraviolet-visible spectroscopy (UV-vis), Fourier Transform Infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), photoluminescence, point of zero-charge (pHpzc), and chromaticity studies. We verified that the ZnO NPs had an average diameter of 6 nm, with a wurtzite crystalline structure, and when excited at 320 nm emitted radiation in the blue region. The methodology for AA detection is based on the observed increase in fluorescence of the molecule complex formed on the ZnO NPs surface after 20 min of interaction. The results indicated that the proposed technique of analysis is fast, simple, and highly sensitive, with a detection limit for AA of 5.15 μM. Furthermore, the nanoparticles presented excellent photostability for at least 30 days, and low sensitivity to other biological organic molecules. The green ZnO NPs also exhibited an efficient response to the presence of AA in actual complex samples, suggesting that the platform here proposed can find use in clinical analysis protocols.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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