Yinghao Liu, Liping Gao, Can Wang, Zunzun Fu, Ran Chen, Weitao Jiang, Chengmiao Yin, Zhiquan Mao, Yanfang Wang
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引用次数: 0
Abstract
Apple replant disease (ARD) negatively affects plant growth and reduces yields in replanted orchards. In this study, biochar and humic acid were applied to apple replant soil. We aimed to investigate whether biochar and humic acid could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms, changing soil microbial community structure, and improving the soil environment. This experiment included five treatments: apple replant soil (CK), apple replant soil with methyl bromide fumigation (FM), replant soil with biochar addition (2 %), replant soil with humic acid addition (1.5 ‰), and replant soil with biochar combined with humic acid. Seedling biomass, the activity of antioxidant enzymes in the leaves and roots, and soil environmental variables were measured. Microbial community composition and structure were analyzed using ITS gene sequencing. Biochar and humic acid significantly reduced the abundance of Fusarium and promoted the recovery of replant soil microbial communities. Biochar and humic acid also increased the soil enzymes activity (urease, invertase, neutral phosphatase, and catalase), the plant height, fresh weight, dry weight, the activity of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), and root indexes of apple seedlings increased in replant soil. In sum, We can use biochar combined with humic acid to alleviate apple replant disease.
苹果移栽病(ARD)会对移栽果园的植物生长造成负面影响,并降低产量。本研究在苹果移栽土壤中施用了生物炭和腐植酸。我们的目的是研究生物炭和腐植酸是否能通过减少有害土壤微生物的生长、改变土壤微生物群落结构和改善土壤环境来促进植物生长和减轻苹果移栽病。本实验包括五个处理:苹果移栽土壤(CK)、苹果移栽土壤加溴甲烷熏蒸(FM)、移栽土壤加生物炭(2 %)、移栽土壤加腐植酸(1.5 ‰)、移栽土壤加生物炭和腐植酸。测量了幼苗生物量、叶片和根部抗氧化酶的活性以及土壤环境变量。利用 ITS 基因测序分析了微生物群落的组成和结构。生物炭和腐植酸大大降低了镰刀菌的数量,促进了移栽土壤微生物群落的恢复。生物炭和腐植酸还提高了土壤酶(脲酶、转化酶、中性磷酸酶和过氧化氢酶)的活性,增加了苹果幼苗在移栽土壤中的株高、鲜重、干重、抗氧化酶(超氧化物歧化酶、过氧化物酶和过氧化氢酶)的活性和根系指数。总之,我们可以利用生物炭与腐植酸的结合来缓解苹果移栽病害。
期刊介绍:
Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.