Maize mutant screens: from classical methods to new CRISPR‐based approaches

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-08-30 DOI:10.1111/nph.20084

Christian Damian Lorenzo, David Blasco‐Escámez, Arthur Beauchet, Pieter Wytynck, Matilde Sanches, Jose Rodrigo Garcia del Campo, Dirk Inzé, Hilde Nelissen

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Abstract

SummaryMutations play a pivotal role in shaping the trajectory and outcomes of a species evolution and domestication. Maize (Zea mays) has been a major staple crop and model for genetic research for more than 100 yr. With the arrival of site‐directed mutagenesis and genome editing (GE) driven by the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), maize mutational research is once again in the spotlight. If we combine the powerful physiological and genetic characteristics of maize with the already available and ever increasing toolbox of CRISPR‐Cas, prospects for its future trait engineering are very promising. This review aimed to give an overview of the progression and learnings of maize screening studies analyzing forward genetics, natural variation and reverse genetics to focus on recent GE approaches. We will highlight how each strategy and resource has contributed to our understanding of maize natural and induced trait variability and how this information could be used to design the next generation of mutational screenings.

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玉米突变体筛选：从经典方法到基于 CRISPR 的新方法

摘要突变在塑造物种进化和驯化的轨迹和结果方面起着举足轻重的作用。100 多年来，玉米（Zea mays）一直是主要的主粮作物和遗传研究的典范。随着定点突变和基因组编辑（GE）技术的出现，玉米突变研究再次成为人们关注的焦点。如果我们把玉米强大的生理和遗传特性与已经可用且不断增加的 CRISPR-Cas 工具箱结合起来，玉米未来的性状工程前景将非常广阔。本综述旨在概述分析正向遗传学、自然变异和反向遗传学的玉米筛选研究的进展和经验，重点介绍最近的基因工程方法。我们将重点介绍每种策略和资源如何促进我们对玉米天然和诱导性状变异的了解，以及如何利用这些信息设计下一代突变筛选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.

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