Reversibly photoswitchable protein assemblies with collagen affinity for in vivo photoacoustic imaging of tumors

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2024-08-30 DOI:10.1126/sciadv.adn8274

Shuai Chen, Kaixin Li, Xin Chen, Shan Lei, Jing Lin, Peng Huang

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Abstract

Recent advancements in photoacoustic (PA) imaging have leveraged reversibly photoswitchable chromophores, known for their dual absorbance states, to enhance imaging sensitivity through differential techniques. Yet, their deployment in tumor imaging has faced obstacles in achieving targeted delivery with high efficiency and specificity. Addressing this challenge, we introduce innovative protein assemblies, DrBphP-CBD, by genetically fusing a photosensory module from Deinococcus radiodurans bacterial phytochrome (DrBphP) with a collagen-binding domain (CBD). These protein assemblies form sub-100-nanometer structures composed of 24 DrBphP dimers and 12 CBD trimers, presenting 24 protein subunits. Their affinity for collagens, combined with impressive photoswitching contrast, markedly improves PA imaging precision. In various tumor models, intravenous administration of DrBphP-CBD has demonstrated enhanced tumor targeting and retention, augmenting contrast in PA imaging by minimizing background noise. This strategy underscores the clinical potential of DrBphP-CBD as PA contrast agents, propelling photoswitchable chromoproteins to the forefront of precise cancer diagnosis.

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具有胶原亲和性的可逆光开关蛋白质组合物，用于肿瘤的活体光声成像

光声（PA）成像的最新进展是利用可逆光开关发色团（以其双重吸收状态而闻名），通过差异技术提高成像灵敏度。然而，它们在肿瘤成像中的应用在实现高效率和特异性的靶向递送方面遇到了障碍。为了应对这一挑战，我们引入了创新性的蛋白质组合--DrBphP-CBD，通过基因融合将放射球菌细菌植物色素（DrBphP）的光敏模块与胶原结合域（CBD）结合在一起。这些蛋白质组合体形成了亚 100 纳米结构，由 24 个 DrBphP 二聚体和 12 个 CBD 三聚体组成，呈现出 24 个蛋白质亚基。它们对胶原蛋白的亲和力与令人印象深刻的光开关对比度相结合，显著提高了 PA 的成像精度。在各种肿瘤模型中，静脉注射 DrBphP-CBD 已显示出更强的肿瘤靶向性和保留性，通过最大限度地减少背景噪声来增强 PA 成像的对比度。这一策略强调了 DrBphP-CBD 作为 PA 造影剂的临床潜力，将光电开关色蛋白推向了癌症精确诊断的前沿。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.