Novel Honeycomb Nanoclay Frameworks With Hemostatic and Antibacterial Properties

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Mélyssa Cambronel, Kan Wongkamhaeng, Christelle Blavignac, Christiane Forestier, Jean-Marie Nedelec, Isabelle Denry
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Abstract

Our laboratory recently developed a new class of high surface area, honeycomb Nanoclay Microsphere Framework absorbents (NMFs) that prompt rapid hemostasis. In the present work, we propose a novel approach to develop antibacterial Topical Hemostatic Agents (THAs) by anchoring silver nanoparticles (AgNPs) onto NMFs. This combination was obtained by a chemical co-reduction approach, followed by freeze-processing, and was shown to ensure stability and on-site delivery of AgNPs, without altering the hemostatic properties of NMFs. Silver-loaded NMFs showed no change in their unique architecture and led to a 55% increase in clot strength, compared to standard control plasma or commercially available THA, and a significant decrease in mean fibrin fiber diameter. Silver nanoparticles were successfully released when solubilized and prevented the growth of both Pseudomonas aeruginosa and Staphylococcus aureus at concentrations of 22 and 30 ppm of silver released, respectively. Overall, cell mortality was between 9.1 ± 5.1% and 6.3 ± 3.2%, depending on AgNP concentration, confirming a low cytotoxicity. Silver-loaded nanoclay microsphere frameworks appear to constitute promising candidates as topical hemostatic agents for secondary management of hemostasis when infection control is needed.

Abstract Image

具有止血和抗菌特性的新型蜂窝状纳米粘土框架
我们的实验室最近开发出了一种新型的高比表面积蜂窝状纳米粘土微球框架吸收剂(NMFs),可促进快速止血。在本研究中,我们提出了一种新方法,通过在 NMFs 上锚定银纳米粒子 (AgNPs),开发抗菌局部止血剂 (THAs)。这种组合是通过化学共还原法获得的,然后进行冷冻处理,结果表明,这种组合能确保银纳米粒子的稳定性和现场递送,同时不改变 NMF 的止血特性。与标准对照血浆或市售 THA 相比,载银 NMF 的独特结构没有发生变化,凝块强度提高了 55%,平均纤维蛋白纤维直径显著减小。纳米银颗粒在溶解后成功释放,并能阻止铜绿假单胞菌和金黄色葡萄球菌的生长,释放的银浓度分别为 22ppm 和 30ppm。总体而言,细胞死亡率介于 9.1 ± 5.1% 和 6.3 ± 3.2% 之间,具体取决于 AgNP 的浓度,这证明其细胞毒性较低。载银纳米粘土微球框架似乎有望成为局部止血剂,在需要控制感染时用于止血的二次管理。
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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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