Vincent Charron-Lamoureux, Sandrine Lebel-Beaucage, Maude Pomerleau, Pascale B. Beauregard
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引用次数: 0
Abstract
Many strains from the Bacillus subtilis species complex exert strong plant growth-promoting activities. However, their efficacy in relevant conditions is variable, due in part to their inability to establish a strong interaction with roots in stressful environmental conditions. Adaptative laboratory evolution (ALE) is a powerful tool to generate novel strains with traits of interest. Many Bacillus evolved isolates, stemming from ALE performed with plants, possess a stronger root colonization capacity. An in-depth analysis of these isolates also allowed the identification of key features influencing the interaction with plant roots. However, many variables can influence the outcome of these assays, and thus, caution should be taken when designing ALE destined to generate better root colonizers.
枯草芽孢杆菌中的许多菌株都具有很强的促进植物生长的活性。然而，它们在相关条件下的功效却不尽相同，部分原因是它们无法在胁迫环境条件下与根系建立强有力的相互作用。实验室适应性进化（ALE）是产生具有相关性状的新型菌株的有力工具。许多芽孢杆菌的进化分离物都来自与植物的适应性进化，具有更强的根定植能力。对这些分离株的深入分析还有助于确定影响与植物根系相互作用的关键特征。然而，许多变量都会影响这些试验的结果，因此在设计 ALE 以产生更好的根定植者时应谨慎行事。
期刊介绍：
Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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