3-Hydroxypropionate production from myo-inositol by the gut acetogen Blautia schinkii

IF 4.3 2区 生物学 Q2 MICROBIOLOGY
Raphael Trischler, Stefanie M. Rustler, Anja Poehlein, Rolf Daniel, Milena Breitenbach, Eric J. N. Helfrich, Volker Müller
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Abstract

Species of the genus Blautia are not only abundant in the human gut but also contribute to human well-being. Our study demonstrates that the gut acetogen Blautia schinkii can grow on myo-inositol. We identified the pathway of myo-inositol degradation through a combination of physiological and biochemical studies, genome-wide expression profiling and homology searches. Initially, myo-inositol is oxidized to 2-keto-myo-inositol. This compound is then metabolized by a series of enzymes – a dehydratase, hydrolase, isomerase and kinase – to form 2-deoxy-5-keto-d-gluconic acid 6-phosphate. This intermediate is split by an aldolase into malonate semialdehyde and dihydroxyacetone phosphate, which is an intermediate of the Embden–Meyerhof–Parnas pathway. This pathway leads to the production of pyruvate and, subsequently, acetate. Concurrently, malonate semialdehyde is reduced to 3-hydroxypropionate (3-HP). The genes responsible for myo-inositol degradation are clustered on the genome, except for the gene encoding the aldolase. We identified the putative aldolase Fba_3 and 3-HP dehydrogenase Adh1 encoding genes bioinformatically and verified them biochemically using enzyme assays with heterologously produced and purified protein. The major fermentation end products were 3-HP and acetate, produced in similar amounts. The production of the unusual fermentation end product 3-HP is significant not only for human health but also for the potential bioindustrial production of this highly desired compound.

Abstract Image

Abstract Image

肠道乙酰菌 Blautia schinkii 从肌醇中产生 3-羟基丙酸盐
布劳氏菌属的物种不仅在人类肠道中大量存在,而且还对人类的健康做出了贡献。我们的研究证明,肠道乙酰原布劳氏菌(Blautia schinkii)可以在肌醇上生长。我们通过生理学和生物化学研究、全基因组表达谱分析和同源性搜索,确定了肌醇降解的途径。肌醇最初被氧化成 2-酮-肌醇。然后,这种化合物通过一系列酶--脱水酶、水解酶、异构酶和激酶--进行代谢,形成 2-脱氧-5-酮基葡萄糖酸 6-磷酸。这种中间体被醛缩酶分解成丙二酸半醛和磷酸二羟丙酮,后者是恩伯登-梅耶霍夫-帕尔纳斯途径的中间体。该途径可产生丙酮酸,随后产生乙酸。同时,丙二酸半醛被还原成 3-羟基丙酸(3-HP)。除了编码醛缩酶的基因外,负责肌醇降解的基因都集中在基因组上。我们通过生物信息学方法确定了推测的醛缩酶 Fba_3 和 3-HP 脱氢酶 Adh1 编码基因,并利用异源生产和纯化的蛋白质进行酶测定,对其进行了生化验证。主要的发酵终产物是 3-HP 和乙酸酯,产量相似。不寻常的发酵终产物 3-HP 的产生不仅对人类健康具有重要意义,而且对这种非常需要的化合物的潜在生物工业生产也具有重要意义。
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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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