Antarctic Streptomyces: Promising biocontrol agents for combating Fusarium oxysporum f. sp. cubense

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2024-09-01 DOI:10.1016/j.btre.2024.e00852

Jeffrey Vargas Perez , Lizette Serrano , Rafael Viteri , Daynet Sosa , Christian A. Romero , Nardy Diez

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Abstract

Fusarium wilt of Banana (FWB) caused by Fusarium oxysporum f. sp. cubense (Foc) poses a significant threat to the banana industry, with current inadequate control measures. This study evaluated the antifungal potential of nine Streptomyces strains isolated from Antarctic soil samples, using Casein-Starch media to stimulate the production of antifungal compounds. The inhibition spectrum against Foc was assessed under laboratory conditions using the well diffusion on Mueller-Hinton agar, with antifungal activity measured in arbitrary units (AU/mL) and minimum inhibitory concentration (MIC) tested using ethyl acetate extracts. Among the nine isolates, K6 and E7 were closely related to Streptomyces polyrhachis and Streptomyces fildesensis, exhibited significant antifungal activity, with K6 and E7 showing 320 and 80 AU/mL, and MIC values of 250 and >500 ppm, respectively. These findings highlight K6 and E7 as potential biocontrol agents against Foc, offering new avenues for sustainable Fusarium wilt management in banana cultivation.

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南极链霉菌：有望防治立方镰刀菌的生物控制剂

由 Fusarium oxysporum f. sp. cubense（Foc）引起的香蕉镰刀菌枯萎病（FWB）对香蕉产业构成了严重威胁，目前的控制措施还不够完善。本研究利用酪蛋白-淀粉培养基刺激抗真菌化合物的产生，评估了从南极土壤样本中分离出的九株链霉菌的抗真菌潜力。在实验室条件下，使用穆勒-欣顿琼脂上的井扩散法评估了对 Foc 的抑制谱，抗真菌活性以任意单位（AU/mL）计算，并使用乙酸乙酯提取物测试了最低抑制浓度（MIC）。在九个分离物中，K6 和 E7 与多血清链霉菌（Streptomyces polyrhachis）和菲尔德斯链霉菌（Streptomyces fildesensis）亲缘关系密切，具有显著的抗真菌活性，K6 和 E7 的抗真菌活性分别为 320 和 80 AU/mL，MIC 值分别为 250 和 500 ppm。这些发现凸显了 K6 和 E7 作为潜在生物控制剂对镰刀菌枯萎病的作用，为香蕉种植中镰刀菌枯萎病的可持续管理提供了新途径。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.