Fibroblast growth factor 8 promotes in vitro neurite outgrowth of placode-derived petrosal and nodose ganglia to varying degrees

IF 2 3区 医学 Q2 ANATOMY & MORPHOLOGY
Peng Zhou , Longfei Cheng , Hengxun Tao , Maik Hintze , Yajun Wang , Qin Pu , Xufeng Qi , Dongqing Cai , Stefanie Kuerten , Jianlin Wang , Ruijin Huang
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Abstract

Fibroblast growth factors (FGFs) are required for the specification and formation of the epibranchial placodes, which give rise to the distal part of the cranial sensory ganglia. However, it remains unclear whether FGFs play a role in regulating the neurite outgrowth of the epibranchial placode-derived ganglia during further development. Previous studies have shown that Fibroblast growth factor 8 (FGF8) promotes neurite outgrowth from the statoacoustic ganglion in vitro. However, these studies did not distinguish between the neural crest- and placode-derived components of the sensory ganglia. In this study, we focused on the petrosal and nodose ganglia as representatives of the epibranchial ganglia and investigated their axonal outgrowth under the influence of FGF8 signaling protein in vitro. To precisely isolate the placode-derived ganglion part, we labeled the placode and its derivatives with enhanced green fluorescent protein (EGFP) through electroporation. The isolated ganglia were then collected for qRT-PCR assay and cultured in a collagen gel with and without FGF8 protein. Our findings revealed that both placode-derived petrosal and nodose ganglia expressed FGFR1 and FGFR2. In culture, FGF8 exerted a neural trophic effect on the axon outgrowth of both ganglia. While the expression levels of FGFR1/2 were similar between the two ganglia, the petrosal ganglion exhibited greater sensitivity to FGF8 compared to the nodose ganglion. This indicates that the placode-derived ganglia have differential responsiveness to FGF8 signaling during axonal extension. Thus, FGF8 is not only required for the early development of the epibranchial placode, as shown in previous studies, but also promotes neurite outgrowth of placode-derived ganglia.

成纤维细胞生长因子 8 在不同程度上促进胎座源性瓣神经节和结节神经节的体外神经元生长
成纤维细胞生长因子(FGFs)是上颅胎座的规格化和形成所必需的,上颅胎座产生了颅感觉神经节的远端部分。然而,FGFs 是否在上颅底神经节的进一步发育过程中起到调节神经元生长的作用仍不清楚。之前的研究表明,成纤维细胞生长因子8(FGF8)可促进体外听神经节神经元的生长。然而,这些研究并未区分感觉神经节的神经嵴和胎座源性成分。在本研究中,我们重点研究了作为外支神经节代表的瓣神经节和结节神经节,并在体外研究了它们在FGF8信号蛋白影响下的轴突生长情况。为了精确分离胎座神经节,我们通过电穿孔用增强型绿色荧光蛋白(EGFP)标记了胎座及其衍生物。然后收集分离出的神经节进行qRT-PCR检测,并在含有或不含FGF8蛋白的胶原凝胶中进行培养。我们的研究结果表明,胎座衍生的瓣神经节和结节神经节都表达了FGFR1和FGFR2。在培养过程中,FGF8对这两个神经节的轴突生长具有神经营养作用。虽然两个神经节的FGFR1/2表达水平相似,但与结节神经节相比,瓣神经节对FGF8表现出更高的敏感性。这表明,在轴突延伸过程中,胎座源性神经节对 FGF8 信号的敏感性有所不同。因此,FGF8不仅是上鳃胎座早期发育所必需的,而且还能促进胎座衍生神经节的神经元生长。
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来源期刊
Annals of Anatomy-Anatomischer Anzeiger
Annals of Anatomy-Anatomischer Anzeiger 医学-解剖学与形态学
CiteScore
4.40
自引率
22.70%
发文量
137
审稿时长
33 days
期刊介绍: Annals of Anatomy publish peer reviewed original articles as well as brief review articles. The journal is open to original papers covering a link between anatomy and areas such as •molecular biology, •cell biology •reproductive biology •immunobiology •developmental biology, neurobiology •embryology as well as •neuroanatomy •neuroimmunology •clinical anatomy •comparative anatomy •modern imaging techniques •evolution, and especially also •aging
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