Evaluation of VITEK MS Version 3.0 MALDI-TOF for the identification of anaerobes, mycobacteria, Nocardia, and moulds

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-08-15 DOI:10.1016/j.diagmicrobio.2024.116477

Kusum Sharma , Archana Angrup , Anup Ghosh , Shreya Singh , Anshul Sood , Amit Arora , Megha Sharma , Sunil Sethi , Shivaprakash M Rudramurthy , Harsimran Kaur , Pallab Ray , Arunaloke Chakrabarti

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引用次数: 0

Abstract

Purpose

The identification of anaerobes, Mycobacterium and Nocardia species, and moulds by MALDI-TOF-MS remains a challenge. This study aimed to evaluate the performance of MALDI-TOF in the identification of these organisms.

Methods

A total of 382 strains, comprising 128 (33.5 %) anaerobes, 126(33.0 %) mycobacterial, 113(29.6 %), mycelial fungi, and 15(3.9 %) Nocardia species were evaluated by VITEK MS Version 3.0. The results were compared with the identification of the isolates by DNA sequence analysis. The DNA sequences used for analysis were the 16S rRNA for anaerobic bacteria, hsp65 gene for mycobacteria, whereas both 16S rRNA and hsp65 gene for Nocardia species, and internal transcribed spacer (ITS) and 28S rRNA gene's D1/D2 regions of fungi.

Results

The VITEK-MS accurately identified 78.3 % (299/382) of the strains at the species, and 9.4 % (36/382) at the genus level. Misidentifications were observed in 3.9 % (15/382) isolates. Of isolates tested, 8.4 % (32/382) were not identified by the system, and 7.06 % (27/382) were not included in the IVD database.

Conclusion

An upgraded VITEK MS V3.0 database provides reasonably accurate and rapid identification of clinically relevant anaerobes, mycobacteria, Nocardia species, and moulds to the species level.

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VITEK MS 3.0 版 MALDI-TOF 鉴定厌氧菌、分枝杆菌、诺卡氏菌和霉菌的评估

目的通过 MALDI-TOF-MS 鉴定厌氧菌、分枝杆菌、诺卡氏菌和霉菌仍然是一项挑战。本研究旨在评估 MALDI-TOF 在鉴定这些生物体方面的性能。方法 VITEK MS 3.0 版共对 382 株菌株进行了评估，其中包括 128 株（33.5%）厌氧菌、126 株（33.0%）分枝杆菌、113 株（29.6%）菌丝真菌和 15 株（3.9%）诺卡氏菌。结果与通过 DNA 序列分析鉴定分离物的结果进行了比较。用于分析的 DNA 序列包括厌氧菌的 16S rRNA、分枝杆菌的 hsp65 基因、诺卡氏菌的 16S rRNA 和 hsp65 基因以及真菌的内部转录间隔（ITS）和 28S rRNA 基因的 D1/D2 区域。3.9%（15/382）的分离株被错误识别。结论升级后的 VITEK MS V3.0 数据库能相当准确、快速地鉴定临床相关厌氧菌、分枝杆菌、诺卡氏菌和霉菌的种级。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.