Turbolysis: A low-cost, small footprint alternative to commercial bead beaters for cell lysis

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC

HardwareX Pub Date : 2024-09-01 DOI:10.1016/j.ohx.2024.e00576

Jason D. Limberis, John Z. Metcalfe

引用次数: 0

Abstract

turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of Mycobacterium tuberculosis roughly correlated to the bacterial load commonly seen in patient samples. turboLysis performed similarly to the BeadBug at low bacterial concentrations and outperformed it at high concentrations above 2x10⁵ CFU/ml (p < 0.005). Thus, turboLysis offers good cell lytic performance in a small form factor at a low cost.

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透析：用于细胞裂解的商用拍珠机的低成本、小体积替代品

turboLysis 是一种新型机械细胞裂解装置，它利用小珠子有效裂解分枝杆菌、酵母菌和拟南芥等坚韧细胞。我们比较了 turboLysis 和 BeadBug 6 在几种浓度的结核分枝杆菌（与患者样本中常见的细菌量大致相关）中的表现。在细菌浓度较低时，turboLysis 的表现与 BeadBug 相似，而在超过 2x105 CFU/ml 的高浓度时，turboLysis 的表现则优于 BeadBug（p <0.005）。因此，turboLysis 能够以较小的体积和较低的成本提供良好的细胞溶解性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.