Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes.

IF 4.2 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Reproductive Biology and Endocrinology Pub Date : 2024-08-28 DOI:10.1186/s12958-024-01274-y

Weijue Su, Chaochao Gong, Haoyue Zhong, Huaqing Yang, Yuyan Chen, Xiaoyuan Wu, Jing Jin, Haitao Xi, Junzhao Zhao

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Abstract

Background: Assisted reproductive technology (ART) is the most effective method to treat infertility and the pathogenesis of implantation failure after in vitro fertilization-embryo transfer (IVF-ET) is a challenging filed in infertility. Microbes in the female reproductive tract are considered to be associated with gynecological and obstetric diseases. However, its effects on embryo implantation failure are unsured.

Purpose: This study aimed to investigate reproductive tract dysbiosis, identify different bacteria in reproductive tract as potential biomarkers of embryo implantation failure and demonstrate the pathogenesis through metabolites analysis.

Methods: We compared the data from 16S rRNA gene and metagenome in reproductive tracts through QIIME2 and HUMAnN2 by the times of embryo implantation failure on 239 infertile patients and 17 healthy women.

Results: Our study revealed a strong positive correlation between Lactobacillus abundance and embryo implantation success (IS) after IVF-ET. The microbial community composition and structure in reproductive tract showed substantially difference between the embryo implantation failure (IF) and healthy control. Moreover, we established a diagnostic model through receiver operating characteristic (ROC) with 0.913 area under curve (AUC) in IS and multiple implantation failures (MIF), verified its effectiveness with an AUC = 0.784 demonstrating microbial community alterations could efficiently discriminate MIF patients. Metagenome functional analyses of vaginal samples from another independent infertile patients after IVF-ET revealed the L-lysine synthesis pathway enriched in IF patients, along with ascended vaginal pH and decreased Lactobacillus abundance.

Conclusions: This study clarifies several independent relationships of bacteria in vagina and endometrial fluid on embryo implantation failure and undoubtedly broadens the understanding about female reproductive health.

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与胚胎移植不良结果相关的阴道和子宫内膜微生物组失调。

背景：辅助生殖技术（ART）是治疗不孕症最有效的方法，而体外受精-胚胎移植（IVF-ET）后着床失败的发病机制是不孕症研究中的一个难题。女性生殖道中的微生物被认为与妇产科疾病有关。目的：本研究旨在调查生殖道菌群失调，确定生殖道中的不同细菌作为胚胎植入失败的潜在生物标志物，并通过代谢物分析证明其发病机制：方法：我们通过 QIIME2 和 HUMAnN2 比较了 239 名不孕患者和 17 名健康女性胚胎植入失败时间生殖道中 16S rRNA 基因和元基因组的数据：我们的研究表明，乳酸杆菌的丰度与体外受精-胚胎着床（IVF-ET）后的胚胎着床成功率（IS）有很强的正相关性。生殖道微生物群落的组成和结构显示，胚胎植入失败（IF）与健康对照之间存在很大差异。此外，我们还通过接收者操作特征（ROC）建立了一个诊断模型，其对IS和多次植入失败（MIF）的曲线下面积（AUC）为0.913，并以AUC = 0.784验证了其有效性，表明微生物群落的改变能有效区分MIF患者。对 IVF-ET 后另一名独立不孕患者的阴道样本进行的元基因组功能分析显示，IF 患者的 L-赖氨酸合成途径丰富，同时阴道 pH 值升高，乳酸杆菌丰度降低：这项研究阐明了阴道和子宫内膜液中的细菌与胚胎植入失败的几种独立关系，无疑拓宽了人们对女性生殖健康的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive Biology and Endocrinology 医学-内分泌学与代谢

CiteScore

7.90

自引率

2.30%

发文量

161

审稿时长

4-8 weeks

期刊介绍： Reproductive Biology and Endocrinology publishes and disseminates high-quality results from excellent research in the reproductive sciences. The journal publishes on topics covering gametogenesis, fertilization, early embryonic development, embryo-uterus interaction, reproductive development, pregnancy, uterine biology, endocrinology of reproduction, control of reproduction, reproductive immunology, neuroendocrinology, and veterinary and human reproductive medicine, including all vertebrate species.