Shotgun metagenomic analysis reveals the emergence of plasmid-encoded mcr-5.1 gene in hospital wastewater in Bangladesh

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
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Abstract

Colistin is considered the last line therapy for treating multidrug-resistant (MDR) bacterial infections in humans. Therefore, the spread of colistin resistance poses a serious threat to human, and environmental health. Though Bangladesh is known as a hotspot of AMR, limited studies have been carried out regarding the status of colistin resistance. Information on the emerging bacterial resistance is inevitable for protecting public health. Nowadays, wastewater analysis has been prioritized for metagenomics-enabled AMR surveillance. Our study on the metagenomic analysis of untreated hospital effluents first detected the colistin resistance-conferring mcr-5.1 gene in the hospital environment of Bangladesh. Phylogenetic tree and in silico AMR analysis confirmed the detection of this mcr-5 variant, which is located in a plasmid contig. The plasmid was untypeable and belonged to the bacteria from the Enterobacteriaceae family. The mcr-5.1 operon was embedded in a Tn3 transposon, suggesting the mobility of the gene. Tnshfr1 transposon, chromate resistance protein ChrB, DNA invertase hin, and two MFS-type proteins were present in the genetic environment of mcr-5.1. Our findings provide evidence of the occurrence of mcr-5.1 in a hospital environment in Bangladesh, which calls for immediate attention and effective measures to contain the dissemination of colistin resistance in the environment.

射枪元基因组分析发现孟加拉国医院废水中出现了质粒编码的 mcr-5.1 基因。
可乐定被认为是治疗人类耐多药(MDR)细菌感染的最后一种疗法。因此,可乐定耐药性的传播对人类和环境健康构成严重威胁。尽管孟加拉国是众所周知的 AMR 热点国家,但有关可乐定耐药性状况的研究却十分有限。要保护公众健康,就必须了解新出现的细菌耐药性。如今,废水分析已被优先用于元基因组学的 AMR 监测。我们对未经处理的医院污水进行的元基因组分析研究首次在孟加拉国的医院环境中检测到了可乐定耐药性确认基因 mcr-5.1。系统发生树和默观 AMR 分析证实了这一 mcr-5 变异基因的检测结果,该基因位于一个质粒等位基因中。该质粒无法分型,属于肠杆菌科细菌。mcr-5.1 操作子嵌入了一个 Tn3 转座子,这表明该基因具有流动性。在 mcr-5.1 的遗传环境中存在 Tnshfr1 转座子、铬酸盐抗性蛋白 ChrB、DNA 转化酶 hin 和两种 MFS 型蛋白。我们的研究结果提供了孟加拉国医院环境中出现 mcr-5.1 的证据,因此需要立即关注并采取有效措施遏制环境中可乐定耐药性的传播。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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