Engineering proteins with catechol chemistry for biotechnological applications.

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Suryalakshmi Pandurangan, Shanmugam Easwaramoorthi, Niraikulam Ayyadurai
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引用次数: 0

Abstract

Developing proteins with increased chemical space by expanding the amino acids alphabet has been an emerging technique to compete for the obstacle encountered by their need in various applications. 3,4-Dihydroxyphenylalanine (L-DOPA) catecholic unnatural amino acid is abundantly present in mussels foot proteins through post-translational modification of tyrosine to give a strong adhesion toward wet rocks. L-DOPA forms: bidentate coordination, H-bonding, metal-ligand complexes, long-ranged electrostatic, and van der Waals interactions via a pair of donor hydroxyl groups. Incorporating catechol in proteins through genetic code expansion paved the way for developing: protein-based bio-sensor, implant coating, bio-conjugation, adhesive bio-materials, biocatalyst, metal interaction and nano-biotechnological applications. The increased chemical spaces boost the protein properties by offering a new chemically active interaction ability to the protein. Here, we review the technique employed to develop a genetically expanded organism with catechol to provide novel properties and functionalities; and we highlight the importance of L-DOPA incorporated proteins in biomedical and industrial fields.

利用邻苯二酚化学成分改造蛋白质,促进生物技术应用。
通过扩展氨基酸字母表来开发具有更大化学空间的蛋白质已成为一种新兴技术,以应对其在各种应用中遇到的障碍。通过对酪氨酸进行翻译后修饰,3,4-二羟基苯丙氨酸(L-DOPA)儿茶酚类非天然氨基酸大量存在于贻贝足蛋白质中,使其对潮湿的岩石具有很强的附着力。L-DOPA 通过一对供体羟基形成:双叉配位、H 键、金属配体复合物、长程静电和范德华相互作用。通过扩展遗传密码将邻苯二酚融入蛋白质,为开发基于蛋白质的生物传感器、植入涂层、生物共轭、粘合生物材料、生物催化剂、金属相互作用和纳米生物技术应用铺平了道路。增加的化学空间为蛋白质提供了新的化学活性相互作用能力,从而提高了蛋白质的特性。在此,我们回顾了利用儿茶酚开发基因扩增生物体以提供新特性和功能的技术,并强调了L-DOPA结合蛋白在生物医学和工业领域的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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