{"title":"Solid-state fermentation of Aspergillus sydowii G12, an approach to produce isocorydine","authors":"","doi":"10.1016/j.procbio.2024.08.021","DOIUrl":null,"url":null,"abstract":"<div><p>Isocorydine is an aporphine alkaloid that is mainly produced via chemical synthesis and extracted from plants. Its hydrochloride has been authorized to be a prescription drug for clinical use. This study aims to discover potential alkaloidal metabolites from endophytic fungi associated with the plant. Endophytic fungi were subjected to solid-state fermentation (SSF) to investigate the production of isocorydine. An aporphine alkaloid was obtained from the metabolite of SSF with <em>Aspergillus sydowii</em> G12. Its chemical structure was deduced to be isocorydine according to the spectroscopic data of ESI-MS, <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The fermentation conditions including substrate, fermentation temperature, and time were optimized. As a result, SSF with <em>A. sydowii</em> G12 using potato as substrate at 20 °C for 15 d could produce isocorydine with a high yield of 2.51 ± 0.09 mg/g. This paper is the first to report an approach to producing isocorydine from microorganisms instead of plants.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S135951132400285X","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Isocorydine is an aporphine alkaloid that is mainly produced via chemical synthesis and extracted from plants. Its hydrochloride has been authorized to be a prescription drug for clinical use. This study aims to discover potential alkaloidal metabolites from endophytic fungi associated with the plant. Endophytic fungi were subjected to solid-state fermentation (SSF) to investigate the production of isocorydine. An aporphine alkaloid was obtained from the metabolite of SSF with Aspergillus sydowii G12. Its chemical structure was deduced to be isocorydine according to the spectroscopic data of ESI-MS, 1H and 13C NMR spectroscopy. The fermentation conditions including substrate, fermentation temperature, and time were optimized. As a result, SSF with A. sydowii G12 using potato as substrate at 20 °C for 15 d could produce isocorydine with a high yield of 2.51 ± 0.09 mg/g. This paper is the first to report an approach to producing isocorydine from microorganisms instead of plants.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.