Molecular characterization and expression profiling of 3 beta-hydroxysteroid dehydrogenase and hydroxysteroid 17-beta dehydrogenase in response to HCG stimulation in striped murrel, Channa striata (Bloch, 1793)

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-08-28 DOI:10.1016/j.genrep.2024.102018

Kiran D. Rasal , Sushma Davu , Prachi Asgolkar , Siba Shinde , Pokanti Vinay Kumar , Siyag Dhere , Arpit Acharya , Rajesh Kumar , Arvind Sonwane , Manoj Brahmane , Jitendra Sundaray , Aparna Chaudhari

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引用次数: 0

Abstract

Striped murrel (Channa striata) is highly valued in the Indian subcontinent and Southeast Asia for its nutritional and medicinal benefits. However, the limited availability of high-quality seeds of murrel and the asynchronous maturation of brooders, particularly during peak spawning seasons creates problems in aquaculture. Beta-hydroxysteroid dehydrogenases (β-HSDs) are steroidogenic enzymes involved in the biosynthesis of active steroids in gametogenesis and steroidogenesis. This study aimed to clone and characterize the full-length cDNA sequences of two crucial enzymes, 3-beta hydroxysteroid dehydrogenase (Hsd3β) and 17-beta hydroxysteroid dehydrogenase (Hsd17β). It resulted in full-length cDNA sequences for Hsd3β and Hsd17β with 1101 bp and 771 bp length, respectively, encoding 366 and 256 amino acids. Signal peptide analysis indicated that both proteins are secretory, and hydropathy profiles suggested their hydrophilic nature. Notably, Rossmann-fold NAD(P)(+)-binding domains characteristic of Short-chain dehydrogenases/reductases (SDR) family genes were identified in Hsd3β (between 9-355 aa) and in Hsd17β (between 10-254 aa). Gene expression analyses were performed in testes and ovaries using qPCR at three key stages: preparatory, mature, and 16 h post human chorionic gonadotropin (hCG) injection (16 hpi). Results demonstrated a significant 2-fold upregulation of Hsd17β in mature and 16 hpi testes, while Hsd3β showed a significant 10-fold upregulation in mature testes compared to premature and 16 hpi stages. Ovarian expression of Hsd3β and Hsd17β showed minimal expression upon hCG injection (P < 0.05). These findings contribute to our understanding of Beta-hydroxysteroid dehydrogenases (β-HSDs), providing insights into regulating sex steroid hormone synthesis during the gonadal development of striped murrel.

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3 beta-羟基类固醇脱氢酶和羟基类固醇 17-beta 脱氢酶在条纹短尾鳕（Channa striata）（Bloch，1793 年）HCG 刺激下的分子特征和表达谱分析

花斑鳢（Channa striata）在印度次大陆和东南亚具有很高的营养和药用价值。然而，优质花鲈种子的有限供应和育雏鱼的不同步成熟（尤其是在产卵高峰期）给水产养殖带来了问题。β-羟基类固醇脱氢酶（β-HSDs）是一种类固醇生成酶，参与配子和类固醇生成过程中活性类固醇的生物合成。本研究旨在克隆 3-beta 羟类固醇脱氢酶（Hsd3β）和 17-beta 羟类固醇脱氢酶（Hsd17β）这两种关键酶的全长 cDNA 序列并确定其特征。该研究获得了 Hsd3β 和 Hsd17β 的全长 cDNA 序列，长度分别为 1101 bp 和 771 bp，编码 366 和 256 个氨基酸。信号肽分析表明，这两种蛋白质都具有分泌功能，而亲水性特征则表明它们具有亲水性。值得注意的是，在 Hsd3β（9-355 aa）和 Hsd17β（10-254 aa）中发现了短链脱氢酶/还原酶（SDR）家族基因特有的罗斯曼折叠 NAD(P)(+)结合域。在睾丸和卵巢的三个关键阶段：准备期、成熟期和注射人绒毛膜促性腺激素（hCG）后 16 小时（16 hpi），使用 qPCR 进行了基因表达分析。结果表明，成熟期和 16 hpi 期睾丸中的 Hsd17β 明显上调了 2 倍，而成熟期睾丸中的 Hsd3β 与未成熟期和 16 hpi 期相比则明显上调了 10 倍。注射 hCG 后，卵巢中 Hsd3β 和 Hsd17β 的表达量极小（P < 0.05）。这些发现有助于我们了解β-羟基类固醇脱氢酶（β-HSDs），为条纹短尾鲈性腺发育过程中性激素合成的调控提供了见解。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.