A lateral strip assay for ultrasensitive detection of glyphosate in soybeans and corn†

IF 3.6 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2024-08-26 DOI:10.1039/D4AN00958D

Xuyang Ma, Liqiang Liu, Shanshan Song, Hua Kuang, Chuanlai Xu and Xinxin Xu

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Abstract

Glyphosate (GLY) is widely applied in agriculture and horticulture as a herbicide. The development of genetically modified plants has caused abuse of GLY, with excessive residues potentially causing harm to human health. Consequently, a novel method needs to be built to detect GLY in soybeans and corn. Computer simulation was used to design an excellent hapten which was used to produce an anti-GLY monoclonal antibody (mAb) with outstanding sensitivity and affinity, and its 50%-inhibitory concentration (IC₅₀) was 128.59 ng mL⁻¹. Afterwards, an immunochromatographic assay strip was developed based on the mAb. In soybeans and corn, the visual detection limits were 1 mg kg⁻¹ and 0.2 mg kg⁻¹, while the cut-off values were 50 mg kg⁻¹ and 5 mg kg⁻¹, respectively. The reliability of the strips was proved by the existing methods. Thus, a rapid method to detect GLY residues on-site in soybeans and corn was established.

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用于超灵敏检测大豆和玉米中草甘膦的横向条带检测法

草甘膦（GLY）作为一种除草剂被广泛应用于农业和园艺业。转基因植物的发展造成了 GLY 的滥用，过量的残留可能会对人类健康造成危害。因此，需要建立一种新型方法来检测大豆和玉米中的 GLY。研究人员利用计算机模拟设计了一种优良的合体蛋白，并利用该合体蛋白制备了一种抗 GLY 的单克隆抗体（mAb），该抗体具有出色的灵敏度和亲和力，其 50%抑制浓度（IC50）为 128.59 ng/mL。随后，基于该 mAb 开发了一种免疫层析检测条。在大豆和玉米中，目测检测限分别为 1 毫克/千克和 0.2 毫克/千克，临界值分别为 50 毫克/千克和 5 毫克/千克。其他现有方法也证明了该试剂条的可靠性。因此，建立了一种现场检测大豆和玉米中 GLY 残留的快速方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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