Surface plasmon polariton–enhanced upconversion luminescence for biosensing applications

IF 6.5 2区物理与天体物理 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanophotonics Pub Date : 2024-08-27 DOI:10.1515/nanoph-2024-0247

Duc Le, Marjut Kreivi, Sanna Aikio, Noora Heinilehto, Teemu Sipola, Jarno Petäjä, Tian-Long Guo, Matthieu Roussey, Jussi Hiltunen

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引用次数: 0

Abstract

Upconversion luminescence (UCL) has great potential for highly sensitive biosensing due to its unique wavelength shift properties. The main limitation of UCL is its low quantum efficiency, which is typically compensated using low-noise detectors and high-intensity excitation. In this work, we demonstrate surface plasmon polariton (SPP)-enhanced UCL for biosensing applications. SPPs are excited by using a gold grating. The gold grating is optimized to match the SPP resonance with the absorption wavelength of upconverting nanoparticles (UCNPs). Functionalized UCNPs conjugated with antibodies are immobilized on the surface of the fabricated gold grating. We achieve an UCL enhancement up to 65 times at low excitation power density. This enhancement results from the increase in the absorption cross section of UCNPs caused by the SPP coupling on the grating surface. Computationally, we investigated a slight quenching effect in the emission process with UCNPs near gold surfaces. The experimental observations were in good agreement with the simulation results. The work enables UCL-based assays with reduced excitation intensity that are needed, for example, in scanning-free imaging.

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用于生物传感应用的表面等离子体极化子增强上转换发光技术

上转换发光（UCL）因其独特的波长偏移特性，在高灵敏度生物传感方面具有巨大潜力。UCL 的主要局限是量子效率低，通常需要使用低噪声探测器和高强度激发来弥补。在这项工作中，我们展示了用于生物传感应用的表面等离子体极化子（SPP）增强型 UCL。SPP 由金光栅激发。金光栅经过优化，使 SPP 共振与上转换纳米粒子（UCNPs）的吸收波长相匹配。与抗体共轭的功能化 UCNPs 被固定在制作好的金光栅表面。在低激发功率密度下，我们实现了高达 65 倍的 UCL 增强。这种增强是由于光栅表面的 SPP 耦合增加了 UCNPs 的吸收截面。我们通过计算研究了 UCNPs 在金表面附近发射过程中的轻微淬火效应。实验观察结果与模拟结果非常吻合。这项研究成果使基于 UCL 的检测方法能够降低激发强度，例如在无扫描成像中就需要这种检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanophotonics NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

13.50

自引率

6.70%

发文量

358

审稿时长

7 weeks

期刊介绍： Nanophotonics, published in collaboration with Sciencewise, is a prestigious journal that showcases recent international research results, notable advancements in the field, and innovative applications. It is regarded as one of the leading publications in the realm of nanophotonics and encompasses a range of article types including research articles, selectively invited reviews, letters, and perspectives. The journal specifically delves into the study of photon interaction with nano-structures, such as carbon nano-tubes, nano metal particles, nano crystals, semiconductor nano dots, photonic crystals, tissue, and DNA. It offers comprehensive coverage of the most up-to-date discoveries, making it an essential resource for physicists, engineers, and material scientists.