Low expression of auxin receptor EcAFB4 confers resistance to florpyrauxifen-benzyl in Echinochloa crus-galli (L.) P. Beauv

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2024-08-24 DOI:10.1016/j.pestbp.2024.106099

Hao Wang , Xiaoxu Li , Yanrong Ren , Haitao Gao , Zhike Feng , Liyao Dong

{"title":"Low expression of auxin receptor EcAFB4 confers resistance to florpyrauxifen-benzyl in Echinochloa crus-galli (L.) P. Beauv","authors":"Hao Wang , Xiaoxu Li , Yanrong Ren , Haitao Gao , Zhike Feng , Liyao Dong","doi":"10.1016/j.pestbp.2024.106099","DOIUrl":null,"url":null,"abstract":"<div>Echinochloa crus-galli (L.) P. Beauv is a monocotyledonous weed that seriously infests rice fields. Florpyrauxifen-benzyl, a novel synthetic auxin herbicide commercialized in China in 2018, is an herbicide for controlling E. crus-galli. However, a suspected resistant population (R) collected in 2012 showed resistance to the previously unused florpyrauxifen-benzyl. Whole-plant dose-response bioassay indicated that the R population evolved high resistance to quinclorac and florpyrauxifen-benzyl. Pretreatment with P450 inhibitors did not influence the GR50 of E. crus-galli to florpyrauxifen-benzyl. The expression of target receptor EcAFB4 was down-regulated in the R population, leading to the reduced response to florpyrauxifen-benzyl (suppresses over-production of ethylene and ABA). We verified this resistance mechanism in the knockout OsAFB4 in Oryza sativa L. The Osafb4 mutants exhibited high resistance to florpyrauxifen-benzyl and moderate resistance to quinclorac. Furthermore, DNA methylation in the EcAFB4 promoter regulated its low expression in the R population after florpyrauxifen-benzyl treatment. In summary, the low expression of the auxin receptor EcAFB4 confers target resistance to the synthetic auxin herbicide florpyrauxifen-benzyl in the R- E. crus-galli.</div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"204 ","pages":"Article 106099"},"PeriodicalIF":4.2000,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357524003328","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Echinochloa crus-galli (L.) P. Beauv is a monocotyledonous weed that seriously infests rice fields. Florpyrauxifen-benzyl, a novel synthetic auxin herbicide commercialized in China in 2018, is an herbicide for controlling E. crus-galli. However, a suspected resistant population (R) collected in 2012 showed resistance to the previously unused florpyrauxifen-benzyl. Whole-plant dose-response bioassay indicated that the R population evolved high resistance to quinclorac and florpyrauxifen-benzyl. Pretreatment with P450 inhibitors did not influence the GR₅₀ of E. crus-galli to florpyrauxifen-benzyl. The expression of target receptor EcAFB4 was down-regulated in the R population, leading to the reduced response to florpyrauxifen-benzyl (suppresses over-production of ethylene and ABA). We verified this resistance mechanism in the knockout OsAFB4 in Oryza sativa L. The Osafb4 mutants exhibited high resistance to florpyrauxifen-benzyl and moderate resistance to quinclorac. Furthermore, DNA methylation in the EcAFB4 promoter regulated its low expression in the R population after florpyrauxifen-benzyl treatment. In summary, the low expression of the auxin receptor EcAFB4 confers target resistance to the synthetic auxin herbicide florpyrauxifen-benzyl in the R- E. crus-galli.

Abstract Image

查看原文本刊更多论文

辅助素受体 EcAFB4 的低表达使 Echinochloa crus-galli (L.) P. Beauv 对氟啶脲苄酯产生抗性

Echinochloa crus-galli (L.) P. Beauv是一种严重危害稻田的单子叶杂草。Florpyrauxifen-benzyl 是一种新型合成助剂除草剂，于 2018 年在中国实现商业化，是一种用于控制 E. crus-galli 的除草剂。然而，2012年收集的一个疑似抗性种群（R）显示出对之前未使用过的氟吡禾草灵-苄的抗性。全株剂量反应生物测定表明，R种群对喹啉草醚和氟螨脲苄酯产生了很强的抗药性。P450 抑制剂的预处理不会影响 E. crus-galli 对氟啶脲苄酯的 GR50。在 R 群体中，靶受体 EcAFB4 的表达下调，导致对氟啶脲苄酯的反应降低（抑制乙烯和 ABA 的过度产生）。我们通过敲除 Oryza sativa L 中的 OsAFB4 验证了这一抗性机制。Osafb4 突变体对氟啶脲苄酯表现出高度抗性，对喹啉酸表现出中度抗性。此外，EcAFB4 启动子中的 DNA 甲基化调节了其在氟吡禾灵-苄基处理后 R 群体中的低表达。总之，辅助素受体 EcAFB4 的低表达使 R- E. crus-galli 对合成辅助素除草剂 florpyrauxifen-benzyl 产生靶向抗性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.