Exploring the protective effects of Qiju Granule in a rat model of dry age-related macular degeneration

IF 3.9

Experimental gerontology Pub Date : 2024-08-27 DOI:10.1016/j.exger.2024.112556

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Abstract

Aim

The aim of this study was to evaluate the potential protective effect of Qiju Granule in a rat model of age-related macular degeneration (AMD) and investigate the underlying mechanisms involved.

Methods

Rats were injected intravenously with 40 mg/kg of sodium iodate (SI) to induce a dry AMD model. The rats in the treatment group received three different doses of Qiju Granule once a day via gavage, while the rats in the control group were given an equal volume of physiological saline. On day 14 and day 28 following the intervention, various methods were employed to evaluate retinal function and structure, including electroretinography (ERG), optical coherence tomography (OCT), and histological examination. The expression of glial fibrillary acidic protein (GFAP), basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) was assessed via immunofluorescence. Beyond immunofluorescence, the mRNA levels of bFGF, BDNF, and CNTF were quantitatively determined using real-time polymerase chain reaction (qRT-PCR).

Results

Rats treated with Qiju Granule exhibited significant improvements in both retinal function and structure compared to the model group. The most noteworthy effects were observed at a high dose of Qiju Granule. Furthermore, the expression levels of bFGF, BDNF, and CNTF were significantly unregulated in the treated groups compared to the model group.

Conclusions

Qiju Granule demonstrated a protective effect on the retina in the SI-induced rat model of AMD. The protective mechanism may be attributed to the upregulation of retinal neurotrophic factors expression.

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探索杞菊颗粒对干性老年性黄斑变性大鼠模型的保护作用

方法大鼠静脉注射40毫克/千克碘酸钠（SI）诱导干性AMD模型。治疗组大鼠每天灌胃一次三种不同剂量的杞菊颗粒，对照组大鼠灌胃等量生理盐水。在干预后的第14天和第28天，采用多种方法评估视网膜功能和结构，包括视网膜电图（ERG）、光学相干断层扫描（OCT）和组织学检查。通过免疫荧光评估了神经胶质纤维酸性蛋白（GFAP）、碱性成纤维细胞生长因子（bFGF）、脑源性神经营养因子（BDNF）和睫状肌神经营养因子（CNTF）的表达。除了免疫荧光外，还使用实时聚合酶链式反应（qRT-PCR）定量测定了 bFGF、BDNF 和 CNTF 的 mRNA 水平。高剂量杞菊颗粒的效果最为显著。结论芪菊颗粒对SI诱导的大鼠AMD模型视网膜具有保护作用。结论杞菊颗粒对SI诱导的大鼠AMD模型视网膜具有保护作用，其保护机制可能是由于上调了视网膜神经营养因子的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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